Laboratory of Bioanalysis and Molecular Imaging, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido 060-0812, Japan.
Molecules. 2021 Mar 15;26(6):1618. doi: 10.3390/molecules26061618.
Optical imaging including fluorescence and luminescence is the most popular method for the in vivo imaging in mice. Luminescence imaging is considered to be superior to fluorescence imaging due to the lack of both autofluorescence and the scattering of excitation light. To date, various luciferin analogs and bioluminescence probes have been developed for deep tissue and molecular imaging. Recently, chemiluminescence probes have been developed based on a 1,2-dioxetane scaffold. In this review, the accumulated findings of numerous studies and the design strategies of bioluminescence and chemiluminescence imaging reagents are summarized.
光学成像是最常用于活体成像的方法，包括荧光和发光。由于缺乏自发荧光和激发光的散射，发光成像被认为优于荧光成像。迄今为止，已经开发了各种荧光素类似物和生物发光探针，用于深层组织和分子成像。最近，基于 1,2-二氧杂环乙烷支架开发了化学发光探针。在这篇综述中，总结了大量研究的积累结果和生物发光和化学发光成像试剂的设计策略。
