Sebina Ismail, Biraro Irene A, Dockrell Hazel M, Elliott Alison M, Cose Stephen
Co-infection Studies Programme, Medical Research Council/Uganda Virus Research Institute Uganda Research Unit on AIDS, Entebbe, Uganda.
Co-infection Studies Programme, Medical Research Council/Uganda Virus Research Institute Uganda Research Unit on AIDS, Entebbe, Uganda; Department of Internal Medicine, College of Health Sciences, Makerere University Kampala, Uganda, Kampala, Uganda.
PLoS One. 2014 Sep 5;9(9):e106796. doi: 10.1371/journal.pone.0106796. eCollection 2014.
Accurate biomarkers of Mycobacterium tuberculosis infection activity would significantly improve early diagnosis, treatment and management of M. tuberculosis infection. We hypothesised that circulating B-lymphocytes may be useful biomarkers of tuberculosis (TB) infection status in highly TB-endemic settings. Ex-vivo and in-vitro mycobacteria-specific B-cell ELISPOT assays were used to examine the plasmablast (PB) and memory B-cell (MBC) responses in the peripheral blood of adult, healthy, community controls (n = 151) and of active TB patients (n = 48) living in Uganda. Frequencies of mycobacteria-specific PBs were markedly higher in active TB patients compared to healthy controls, and, conversely, MBCs were markedly higher in the healthy controls compared to active TB patients. In addition, the community controls with evidence of latent TB infection had higher peripheral blood PB and MBC responses than those without evidence of TB infection. These data demonstrate that peripheral blood B-cell responses are differentially modulated during latent and active M. tuberculosis infection, and suggest that the PB to MBC ratio may be a useful biomarker of TB infection activity.
结核分枝杆菌感染活动的准确生物标志物将显著改善结核病感染的早期诊断、治疗和管理。我们假设，在结核病高度流行地区，循环B淋巴细胞可能是结核病（TB）感染状态的有用生物标志物。采用体外和体内分枝杆菌特异性B细胞ELISPOT试验，检测了乌干达成年健康社区对照者（n = 151）和活动性结核病患者（n = 48）外周血中的浆母细胞（PB）和记忆B细胞（MBC）反应。与健康对照相比，活动性结核病患者中分枝杆菌特异性PB的频率显著更高，相反，与活动性结核病患者相比，健康对照中的MBC显著更高。此外，有潜伏性结核感染证据的社区对照者外周血PB和MBC反应高于无结核感染证据者。这些数据表明，在潜伏性和活动性结核分枝杆菌感染期间，外周血B细胞反应受到不同调节，并提示PB与MBC的比值可能是结核病感染活动的有用生物标志物。
