Eau de Paris, DRDQE, R&D biologie, 33, Avenue Jean Jaurès, 94200 Ivry sur Seine, France.
LEESU (UMR MA 102, Université Paris-Est, Agro ParisTech), Université Paris-Est Créteil, 61, avenue du Général-de-Gaulle, 94010 Créteil Cedex, France.
J Virol Methods. 2014 Dec;209:47-54. doi: 10.1016/j.jviromet.2014.08.016. Epub 2014 Sep 6.
Despite the progress in water and wastewater treatment technologies, waterborne diseases are still a major concern of public health. In the reported water-related outbreaks, viruses constitute one of the main causal agents. Enteroviruses are one of the most viruses monitored in water and are often used as an indicator of viral pollution. Isolation and identification of this virus are now regularly based on molecular tools. However published or commercial protocols for detection of these viruses in water are frequently lacking of validation processes and performance evaluation in such complex samples. A method for enterovirus detection in environmental water has been developed, its performance has been evaluated and compared with several commercial kits. The sensitivity of commercial methods in clinical samples, ranged between 89% and 100%, while the sensitivity in seeded environmental matrices fell between 16% and 91%. This method showed the best performance in environmental samples and was subsequently applied on surface and treated wastewater. The results showed the large dissemination of enteroviruses in an urbanized river. The results also emphasized the importance of good knowledge of the method's limits for its utilization in environmental samples in order to minimize false negatives and to avoid underestimating viral concentration.
尽管在水和废水处理技术方面取得了进展，但水传播疾病仍然是公共卫生的主要关注点。在已报告的与水有关的疫情中，病毒是主要病原体之一。肠道病毒是水中监测到的主要病毒之一，通常用作病毒污染的指标。目前，该病毒的分离和鉴定通常基于分子工具。然而，在水质检测中，已发表的或商业性的检测方法通常缺乏验证程序，也未对这些复杂样本的性能进行评估。现已开发出一种用于检测环境水中肠道病毒的方法，并对其性能进行了评估，同时与几种商业试剂盒进行了比较。在临床样本中，商业方法的灵敏度在 89%至 100%之间，而在接种环境基质中的灵敏度在 16%至 91%之间。该方法在环境样本中表现出最佳性能，随后将其应用于地表水和处理后的废水。结果表明，在城市化河流中肠道病毒广泛传播。结果还强调了在环境样本中使用该方法时充分了解方法局限性的重要性，以尽量减少假阴性和避免低估病毒浓度。
