Muluye Dagnachew, Wondimeneh Yitayih, Ferede Getachew, Nega Tesfaye, Adane Kasaw, Biadgo Belete, Tesfa Habtie, Moges Feleke
School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, P,O, Box 196 Gondar, Ethiopia.
BMC Res Notes. 2014 Sep 9;7:619. doi: 10.1186/1756-0500-7-619.
In spite of advances in control of infections, wound infections have not completely controlled due to many reasons. The widespread uses of antibiotics, together with the length of time over which they have been available have led to major problems of resistant organisms contributing to morbidity and mortality. This study was aimed to assess bacterial isolates and their drug susceptibility patterns from patients with pus and/or wound discharge.
A retrospective study was conducted at Gondar University Hospital from all individuals who provide pus and/or wound discharge sample from September, 2009 to August, 2012. Socio-demographic and laboratory results were collected from the University Hospital Microbiology Laboratory unit registration books by using a standard data collection format. Data were analyzed using SPSS version 20 software. P-value ≤ 0.05 was considered statistically significant.
A total of 628 study subjects were included in the study with bacterial isolation rate of 441 (70.2%). Of all, 344 (54.8%) were males. Two hundred eighty two (63.9%) of the isolates were gram positive and 159 (36.1%) were gram negative. About 331/ 441 (75.0%) of the total isolates were Staphylococcus aureus (32.9%), Coagulase Negative staphylococci (14.7%), Streptococcus spp. (11.6%), Escherichia coli (9.5%), Klebsiella spp. (6.3%). The result showed that 66.2% of the isolates were resistant to tetracycline, followed 59.8% for ampicillin, 59.1% for cotrimoxazole, 51.7% for penicillin; least resistant being 6.3% for gentamycin.
High prevalence of bacterial isolates were found; S. aureus being the dominant. Most of the isolates were resistant to many of the antibiotics tested where all isolates of Pseudomonas spp. being resistant to two or more antibiotics. Antibiotic susceptibility test is necessary for effective control of wound infections.
尽管在感染控制方面取得了进展，但由于多种原因，伤口感染尚未得到完全控制。抗生素的广泛使用及其上市时间之长，导致了耐药菌引发的重大问题，增加了发病率和死亡率。本研究旨在评估有脓液和/或伤口分泌物的患者的细菌分离株及其药敏模式。
在贡德尔大学医院对2009年9月至2012年8月期间提供脓液和/或伤口分泌物样本的所有个体进行了一项回顾性研究。通过使用标准数据收集格式，从大学医院微生物学实验室单位登记册中收集社会人口统计学和实验室结果。使用SPSS 20版软件进行数据分析。P值≤0.05被认为具有统计学意义。
共有628名研究对象纳入研究，细菌分离率为441例（70.2%）。其中，344例（54.8%）为男性。分离株中282例（63.9%）为革兰氏阳性菌，159例（36.1%）为革兰氏阴性菌。在所有分离株中，约331/441（75.0%）为金黄色葡萄球菌（32.9%）、凝固酶阴性葡萄球菌（14.7%）、链球菌属（11.6%）、大肠杆菌（9.5%）、克雷伯菌属（6.3%）。结果显示，66.2%的分离株对四环素耐药，其次是氨苄西林59.8%、复方新诺明59.1%、青霉素51.7%；对庆大霉素耐药率最低，为6.3%。
发现细菌分离株的高流行率；金黄色葡萄球菌占主导地位。大多数分离株对多种测试抗生素耐药，所有铜绿假单胞菌分离株均对两种或更多抗生素耐药。药敏试验对于有效控制伤口感染是必要的。
