Nogo-B 受体表达与浸润性导管乳腺癌的恶性程度和 ki-67 抗原表达呈负相关。
Nogo-B receptor expression correlates negatively with malignancy grade and ki-67 antigen expression in invasive ductal breast carcinoma.
机构信息
Department of Histology and Embryology, Medical University, Wroclaw, Poland.
Department of Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Environmental and Life Sciences, Wroclaw, Poland Laboratory of Glycobiology and Cell Interactions, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
出版信息
Anticancer Res. 2014 Sep;34(9):4819-28.
BACKGROUND
Nogo-B receptor (NgBR) has been shown to be involved in endothelial cell chemotaxis and morphogenesis. However, few studies analyzing its expression in cancer cells have been performed.
MATERIALS AND METHODS
We examined NgBR expression in 233 patients with invasive ductal breast carcinoma (IDC) and corresponding non-malignant breast tissues (NMBT) on mRNA (real-time polymerase chain reaction) and protein levels (immunohistochemistry; IHC and western-blot analysis). NgBR expression was found also analyzed in breast cancer cell lines of varying invasiveness.
RESULTS
NgBR expression was increased in IDC compared to NMBT on the mRNA (p=0.0007) and protein level (p=0.018). NgBR expression decreased significantly with IDC malignancy grade and correlated negatively with the Ki-67 antigen expression (r=-0.18; p=0.0005). High NgBR mRNA expression was associated with estrogen receptor negativity (p=0.0023) and the triple-negative phenotype of the tumors (p=0.0129).
CONCLUSION
NgBR may be involved in IDC development, however, its role in its progression requires further research.
背景
Nogo-B 受体（NgBR）已被证明参与内皮细胞趋化和形态发生。然而，很少有研究分析其在癌细胞中的表达。
材料和方法
我们在 233 名浸润性导管乳腺癌（IDC）患者和相应的非恶性乳腺组织（NMBT）上检测了 NgBR 的表达水平，检测方法为信使 RNA（实时聚合酶链反应）和蛋白质水平（免疫组织化学；免疫组化和蛋白质印迹分析）。我们还分析了不同侵袭性的乳腺癌细胞系中的 NgBR 表达情况。
结果
与 NMBT 相比，IDC 中 NgBR 的表达在信使 RNA（p=0.0007）和蛋白质水平（p=0.018）上均升高。NgBR 的表达随 IDC 恶性程度的降低而显著降低，并与 Ki-67 抗原表达呈负相关（r=-0.18；p=0.0005）。高 NgBR 信使 RNA 表达与雌激素受体阴性（p=0.0023）和肿瘤的三阴性表型（p=0.0129）相关。
结论
NgBR 可能参与 IDC 的发展，但它在 IDC 进展中的作用需要进一步研究。
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