Department of Spine, Beijing Jishuitan Hospital, Beijing, China.
Beijing Institute of Orthopedics and Traumatology, Beijing, China.
Neural Regen Res. 2014 May 15;9(10):1014-9. doi: 10.4103/1673-5374.133160.
A novel double-layer collagen membrane with unequal pore sizes in each layer was designed and tested in this study. The inner, loose layer has about 100-μm-diameter pores, while the outer, compact layer has about 10-μm-diameter pores. In a rat model of incomplete spinal cord injury, a large number of neural stem cells were seeded into the loose layer, which was then adhered to the injured side, and the compact layer was placed against the lateral side. The results showed that the transplantation of neural stem cells in a double-layer collagen membrane with unequal pore sizes promoted the differentiation of neural stem cells, attenuated the pathological lesion, and significantly improved the motor function of the rats with incomplete spinal cord injuries. These experimental findings suggest that the transplantation of neural stem cells in a double-layer collagen membrane with unequal pore sizes is an effective therapeutic strategy to repair an injured spinal cord.
本研究设计并测试了一种具有各层不等孔径的新型双层胶原膜。内层疏松层的孔径约为 100μm，而外层致密层的孔径约为 10μm。在不完全性脊髓损伤的大鼠模型中，将大量神经干细胞接种到疏松层中，然后将其贴附在损伤侧，将致密层放置在外侧。结果表明，在具有不等孔径的双层胶原膜中移植神经干细胞可促进神经干细胞的分化，减轻病理性损伤，并显著改善不完全性脊髓损伤大鼠的运动功能。这些实验结果表明，在具有不等孔径的双层胶原膜中移植神经干细胞是修复受损脊髓的一种有效治疗策略。
