Ogawa Toru, Vandamme Katleen, Zhang Xiaolei, Naert Ignace, Possemiers Tine, Chaudhari Amol, Sasaki Keiichi, Duyck Joke
BIOMAT KU Leuven, Department of Oral Health Sciences & Dental Clinic, KU Leuven & University Hospitals Leuven, Kapucijnenvoer 7, P.O. Box 7001, 3000, Leuven, Belgium.
Calcif Tissue Int. 2014 Nov;95(5):467-75. doi: 10.1007/s00223-014-9896-x. Epub 2014 Sep 11.
Low-magnitude high-frequency loading, applied by means of whole body vibration (WBV), affects the bone. Deconstructing a WBV loading stimulus into its constituent elements and investigating the effects of frequency and acceleration individually on bone tissue kinetics around titanium implants were aimed for in this study. A titanium implant was inserted in the tibia of 120 rats. The rats were divided into 1 control group (no loading) and 5 test groups with low (L), medium (M) or high (H) frequency ranges and accelerations [12-30 Hz at 0.3×g (F(L)A(H)); 70-90 Hz at 0.075×g (F(M)A(M)); 70-90 Hz at 0.3×g (F(M)A(H)); 130-150 Hz at 0.043×g (F(H)A(L)); 130-150 Hz at 0.3×g (F H A H)]. WBV was applied for 1 or 4 weeks. Implant osseointegration was evaluated by quantitative histology (bone-to-implant contact (BIC) and peri-implant bone formation (BV/TV)). A 2-way ANOVA (duration of experimental period; loading mode) with α = 0.05 was performed. BIC significantly increased over time and under load (p < 0.0001). The highest BICs were found for loading regimes at high acceleration with medium or high frequency (F(M)A(H) and F(H)A(H)), and significantly differing from F(L)A(H) and F(M)A(M) (p < 0.02 and p < 0.005 respectively). BV/TV significantly decreased over time (p < 0.0001). Loading led to a site-specific BV/TV increase (p < 0.001). The highest BV/TV responses were found for F(M)A(H) and F(H)A(H), significantly differing from F(M)A(M) (p < 0.005). The findings reveal the potential of high-frequency vibration loading to accelerate and enhance implant osseointegration, in particular when applied at high acceleration. Such mechanical signals hold great, though untapped, potential to be used as non-pharmacologic treatment for improving implant osseointegration in compromised bone.
通过全身振动（WBV）施加的低强度高频负荷会影响骨骼。本研究旨在将WBV负荷刺激分解为其组成要素，并分别研究频率和加速度对钛植入物周围骨组织动力学的影响。将一枚钛植入物植入120只大鼠的胫骨中。大鼠被分为1个对照组（无负荷）和5个试验组，试验组具有低（L）、中（M）或高（H）频率范围和加速度[0.3×g下12 - 30Hz（F(L)A(H)）；0.075×g下70 - 90Hz（F(M)A(M)）；0.3×g下70 - 90Hz（F(M)A(H)）；0.043×g下130 - 150Hz（F(H)A(L)）；0.3×g下130 - 150Hz（F(H)A(H)）]。施加WBV 1周或4周。通过定量组织学（骨与植入物接触（BIC）和植入物周围骨形成（BV/TV））评估植入物的骨整合情况。进行α = 0.05的双向方差分析（实验周期时长；负荷模式）。BIC随时间推移和在负荷作用下显著增加（p < 0.0001）。在高加速度下的中高频负荷方案（F(M)A(H)和F(H)A(H)）中发现了最高的BIC，且与F(L)A(H)和F(M)A(M)有显著差异（分别为p < 0.02和p < 0.005）。BV/TV随时间显著降低（p < 0.0001）。负荷导致特定部位的BV/TV增加（p < 0.001）。在F(M)A(H)和F(H)A(H)中发现了最高的BV/TV反应，与F(M)A(M)有显著差异（p < 0.005）。研究结果揭示了高频振动负荷在加速和增强植入物骨整合方面的潜力，特别是在高加速度应用时。这种机械信号具有巨大的、尚未开发的潜力，可作为改善受损骨中植入物骨整合的非药物治疗方法。
