Gunyakti Kilinc Seyma, Kesik Harun Kaya, Celik Figen, Simsek Sami
Department of Parasitology, Faculty of Veterinary Medicine, Bingol University, Bingol, Turkey.
Department of Parasitology, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey.
Vet Med Sci. 2025 Mar;11(2):e70313. doi: 10.1002/vms3.70313.
Echinococcus granulosus is a zoonotic disease that is widespread worldwide.
This study aimed to determine the genetic diversity of E. granulosus isolates in sheep. Partial mitochondrial and nuclear gene sequences were used to determine intraspecific variation.
For this purpose, 41 sheep hydatid cyst isolates were collected from slaughterhouses in Elazig and Bingol provinces of Türkiye. Following genomic DNA isolation from the germinal membrane of the cysts, PCR amplification and subsequent DNA sequence analysis were performed using primers that amplify mitochondrial CO1, Nad5, and nuclear ef1α gene regions.
DNA sequence analyses of mt-CO1 (1603 bp) and mt-Nad5 (625 bp) gene regions showed that 39 out of 41 isolates were identified as G1 and two isolates as G3. When the phylogenies formed by the sequences belonging to different gene regions were evaluated; in the phylogenetic tree created with the mt-CO1 and Nad5 gene dataset, G1 and G3 isolates were in separate clades with the reference sequences, while in the phylogenetic tree created with the nuclear gene region ef1α dataset, the isolate belonging to the G3 genotype (ef1a.01) formed a sister clade with the G1 genotypes and the reference sequence. According to the haplotype network results obtained, 14 haplotypes, 15 haplotypes, and two haplotypes were determined for mt-CO1, mt-Nad5, and ef1α gene regions, respectively. Haplotype analysis of mt-CO1 and mt-Nad5 gene regions revealed high haplotype and low nucleotide diversity. Low nucleotide diversity was detected, and two haplotypes were determined as a result of haplotype analysis ef1α (1343 bp) gene.
细粒棘球绦虫是一种在全球广泛传播的人畜共患病。
本研究旨在确定绵羊体内细粒棘球绦虫分离株的遗传多样性。使用部分线粒体和核基因序列来确定种内变异。
为此，从土耳其埃拉泽省和宾格尔省的屠宰场收集了41份绵羊包虫囊肿分离株。从囊肿的生发膜中分离基因组DNA后，使用扩增线粒体CO1、Nad5和核ef1α基因区域的引物进行PCR扩增及后续DNA序列分析。
mt-CO1（1603 bp）和mt-Nad5（625 bp）基因区域的DNA序列分析表明，41份分离株中有39份被鉴定为G1型，两份分离株为G3型。当评估由不同基因区域的序列形成的系统发育时；在由mt-CO1和Nad5基因数据集创建的系统发育树中，G1和G3分离株与参考序列处于不同的分支中，而在由核基因区域ef1α数据集创建的系统发育树中，属于G3基因型（ef1a.01）的分离株与G1基因型和参考序列形成了一个姐妹分支。根据获得的单倍型网络结果，mt-CO1、mt-Nad5和ef1α基因区域分别确定了14个单倍型、15个单倍型和两个单倍型。mt-CO1和mt-Nad5基因区域的单倍型分析显示单倍型多样性高而核苷酸多样性低。检测到低核苷酸多样性，ef1α（1343 bp）基因的单倍型分析结果确定了两个单倍型。
