Kanwal Sumaira, Perveen Shazia, Hameed Huma, Tahir Sadia, Rashid Rehmana, Raish Mohammad, Bashir Sadia, Zaffar Khudija, Irfan Ali, Kotwica-Mojzych Katarzyna, Faheem Saleha, Ahmad Ajaz, Kashif Muhammad, Bin Jardan Yousef A
COMSATS University Islamabad Sahiwal Campus, Islamabad, Pakistan.
Department of Zoology, The Women University Multan, Multan, Pakistan.
Sci Rep. 2025 May 2;15(1):15406. doi: 10.1038/s41598-025-00127-x.
The relationship between Streptococcus mutans and dental caries has been a key focus of research due to the widespread prevalence of caries in humans. In this study, we have investigated the antimicrobial activity of Chenopodium murale (C. murale) extract against Streptococcus mutans by the application of 10 µL of ethanolic extract. The minimum bactericidal concentration test was used for antimicrobial sensitivity by the application of ethanolic extracts of C. murale. The zone of inhibition diameter made by the extract of C. murale, was measured at 12 mm and compared with the standard reference ampicillin and clindamycin antibiotic drugs, measured at 20 mm and 30 mm, respectively. Moreover, the FTIR analysis showed alkyl halide, phenyl, carbonyl, alkane, amines, and alcohol functional groups in C. murale extract. The C. murale plant extract has significant and comparable therapeutic efficacy with reference to the ampicillin reference drug against the bacterial strain Streptococcus mutans, on account of scavenging bond stretches with fewer side effects owing to its natural origin. Further, presence of coumarin in the pure extract of C. murale was confirmed by TLC method. The anti-Streptococcus mutans activity of the C. murale ethanolic extract was found with the MIC of 1.469 mg/mL and MBC of 4.260 and cariogenic properties of Streptococcus mutans linked to pH drop was also inhibited significantly. Our study clearly indicated that this plant extract thus can potentially be used as a commercialized product in near future for the prevention of dental caries.
由于龋齿在人类中普遍流行，变形链球菌与龋齿之间的关系一直是研究的重点。在本研究中，我们通过应用10微升乙醇提取物，研究了藜（C. murale）提取物对变形链球菌的抗菌活性。采用最小杀菌浓度试验来检测藜乙醇提取物的抗菌敏感性。测量了藜提取物产生的抑菌圈直径为12毫米，并与标准参考氨苄西林和克林霉素抗生素药物进行比较，它们的抑菌圈直径分别为20毫米和30毫米。此外，傅里叶变换红外光谱（FTIR）分析表明藜提取物中存在卤代烷、苯基、羰基、烷烃、胺和醇官能团。藜植物提取物对变形链球菌菌株具有与氨苄西林参考药物相当的显著治疗效果，这归因于其天然来源，能清除键伸缩且副作用较少。此外，通过薄层色谱（TLC）法证实了藜纯提取物中香豆素的存在。发现藜乙醇提取物对变形链球菌的抗活性，其最低抑菌浓度（MIC）为1.469毫克/毫升，最低杀菌浓度（MBC）为4.260，并且与pH下降相关的变形链球菌的致龋特性也受到显著抑制。我们的研究清楚地表明，这种植物提取物在不久的将来有可能作为一种商业化产品用于预防龋齿。
