Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 151 Malianwa North Road, Beijing 100193, China.
Evid Based Complement Alternat Med. 2014;2014:160373. doi: 10.1155/2014/160373. Epub 2014 Aug 19.
The bacterial communities of 1- to 6-year ginseng rhizosphere soils were characterized by culture-independent approaches, random amplified polymorphic DNA (RAPD), and amplified ribosomal DNA restriction analysis (ARDRA). Culture-dependent method (Biolog) was used to investigate the metabolic function variance of microbe living in rhizosphere soil. Results showed that significant genetic and metabolic function variance were detected among soils, and, with the increasing of cultivating years, genetic diversity of bacterial communities in ginseng rhizosphere soil tended to be decreased. Also we found that Verrucomicrobia, Acidobacteria, and Proteobacteria were the dominants in rhizosphere soils, but, with the increasing of cultivating years, plant disease prevention or plant growth promoting bacteria, such as Pseudomonas, Burkholderia, and Bacillus, tended to be rare.
采用非培养方法、随机扩增多态性 DNA(RAPD)和扩增核糖体 DNA 限制分析(ARDRA)对 1 至 6 年人参根际土壤中的细菌群落进行了表征。采用培养依赖方法(Biolog)研究了根际土壤中微生物的代谢功能差异。结果表明,土壤之间存在显著的遗传和代谢功能差异,随着培养年限的增加,人参根际土壤中细菌群落的遗传多样性趋于降低。还发现根际土壤中的优势菌群为疣微菌门、酸杆菌门和变形菌门,但随着培养年限的增加,植物病害防治或促进植物生长的细菌,如假单胞菌、伯克霍尔德菌和芽孢杆菌,趋于减少。