Sadeghi Farzin, Salehi-Vaziri Mostafa, Ghodsi Seyed Mohammad, Alizadeh Ahad, Bokharaei-Salim Farah, Saroukalaei Sedigheh Taghinezhad, Mirbolouk Mohammadhossein, Monavari Seyed Hamidreza, Keyvani Hossein
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Arch Virol. 2015 Jan;160(1):61-8. doi: 10.1007/s00705-014-2230-0. Epub 2014 Sep 14.
The human neurotropic JC virus (JCV) is of significant interest due to its experimental neuro- oncogenic potential. In clinical samples from human central nervous system (CNS) tumors, detection of JCV sequences suggests a possible association with CNS neoplasms, but the results are discrepant worldwide. To assess the prevalence of JCV sequences in Iranian patients with primary and metastatic CNS malignancies, a total of 58 fresh CNS tumors were examined by quantitative real-time PCR targeting the JCV large T antigen (LT-Ag) gene, and JCV DNA load was determined as viral copy number per cell. All patients were immunocompetent, and none of them had received immunosuppressive therapy before surgical operation. JC virus LT-Ag sequences were found in a total of 15 (25.9 %) out of the 58 tested samples. In primary CNS tumors, JCV sequences were identified more frequently in meningiomas (50.0 %) and schwannomas (35.7 %). In metastatic CNS tumors, JCV LT-Ag was identified in one case with brain adenocarcinoma originating from lung cancer. No statistically significant association between JCV positivity and various types of CNS malignancies was observed (P = 0.565). The mean JCV LT-Ag copy number in 15 positive cases was 1.8 × 10(-4) ± 4.5 × 10(-4) copies per cell (range 1.0 × 10(-5)-1.78 × 10(-3) copies per cell). An inverse correlation between white blood cell (WBC) count and JCV copy number was observed, but this correlation was not statistically significant (R = -0.198, P = 0.480). This study provides the first data on the prevalence of JCV in primary and metastatic CNS tumors from Iranian patients.
人类嗜神经JC病毒（JCV）因其潜在的实验性神经致癌能力而备受关注。在人类中枢神经系统（CNS）肿瘤的临床样本中，JCV序列的检测提示其可能与CNS肿瘤有关，但全球范围内的结果并不一致。为评估伊朗原发性和转移性CNS恶性肿瘤患者中JCV序列的流行情况，我们采用靶向JCV大T抗原（LT-Ag）基因的定量实时PCR检测了58例新鲜CNS肿瘤，并将JCV DNA载量确定为每细胞的病毒拷贝数。所有患者免疫功能正常，且术前均未接受免疫抑制治疗。在58个检测样本中，共发现15个（25.9%）样本存在JC病毒LT-Ag序列。在原发性CNS肿瘤中，JCV序列在脑膜瘤（50.0%）和神经鞘瘤（35.7%）中更常见。在转移性CNS肿瘤中，1例源自肺癌的脑腺癌病例检测到JCV LT-Ag。未观察到JCV阳性与各种类型CNS恶性肿瘤之间存在统计学显著关联（P = 0.565）。15例阳性病例中JCV LT-Ag的平均拷贝数为每细胞1.8×10^(-4)±4.5×10^(-4)拷贝（范围为每细胞1.0×10^(-5)-1.78×10^(-3)拷贝）。观察到白细胞（WBC）计数与JCV拷贝数之间呈负相关，但这种相关性无统计学意义（R = -0.198，P = 0.480）。本研究提供了伊朗患者原发性和转移性CNS肿瘤中JCV流行情况的首批数据。
