Renata Hans, Wang Z Jane, Kitto Rebekah Z, Arnold Frances H
Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 E. California Blvd., MC-210-41, Pasadena, CA.
Catal Sci Technol. 2014 Oct 2;4(10):3640-3643. doi: 10.1039/C4CY00633J.
A variant of P450 from five mutations away from wild type is a highly active catalyst for cyclopropanation of a variety of acrylamide and acrylate olefins with ethyl diazoacetate (EDA). The very high rate of reaction enabled by histidine ligation allowed the reaction to be conducted under aerobic conditions. The promiscuity of this catalyst for a variety of substrates containing amides has enabled synthesis of a small library of precursors to levomilnacipran derivatives.
一种与野生型相差五个突变的细胞色素P450变体，是一种高效催化剂，可用于用重氮乙酸乙酯（EDA）对多种丙烯酰胺和丙烯酸酯烯烃进行环丙烷化反应。组氨酸连接实现的极高反应速率使得该反应能够在有氧条件下进行。这种催化剂对多种含酰胺底物的通用性使得能够合成一小批左旋米那普明衍生物的前体文库。
