Fasan Rudi, Jennifer Kan S B, Zhao Huimin
Department of Chemistry, University of Rochester, Rochester, NY 14627, United States.
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, United States.
ACS Catal. 2019 Nov 1;9(11):9775-9788. doi: 10.1021/acscatal.9b02737. Epub 2019 Sep 17.
On the occasion of Professor Frances H. Arnold's recent acceptance of the 2018 Nobel Prize in Chemistry, we honor her numerous contributions to the fields of directed evolution and biocatalysis. Arnold pioneered the development of directed evolution methods for engineering enzymes as biocatalysts. Her highly interdisciplinary research has provided a ground not only for understanding the mechanisms of enzyme evolution but also for developing commercially viable enzyme biocatalysts and biocatalytic processes. In this Account, we highlight some of her notable contributions in the past three decades in the development of foundational directed evolution methods and their applications in the design and engineering of enzymes with desired functions for biocatalysis. Her work has created a paradigm shift in the broad catalysis field.
在弗朗西斯·H·阿诺德教授最近荣获2018年诺贝尔化学奖之际，我们向她在定向进化和生物催化领域的众多贡献致以敬意。阿诺德率先开发了将酶工程改造为生物催化剂的定向进化方法。她极具跨学科性的研究不仅为理解酶进化机制奠定了基础，还为开发具有商业可行性的酶生物催化剂和生物催化工艺提供了支撑。在这篇综述中，我们重点介绍她在过去三十年里在基础定向进化方法开发方面的一些显著贡献，以及这些方法在设计和改造具有所需生物催化功能的酶中的应用。她的工作在广泛的催化领域引发了范式转变。
