Mohapatra Sasmita, Rout Smruti Ranjan, Narayan Rajan, Maiti Tapas K
Department of Chemistry, National Institute of Technology, Rourkela, 769008, India.
Dalton Trans. 2014 Nov 14;43(42):15841-50. doi: 10.1039/c4dt02144d.
The development of theranostic mesoporous hollow silica nanospheres having therapeutic and diagnostic functions has been achieved. The exterior surface of the hollow mesoporous silica nanosphere was selectively acid functionalized and utilised to conjugate the anticancer drug cisplatin, the marker molecule folic acid (FA), and rhodamine isothiocyanate (RITC), whereas the interior space was utilised to encapsulate superparamagnetic CoFe2O4 nanoparticles as well as the hydrophobic anticancer drug pemetrexed. The hydrodynamic size of the synthesized multidrug loaded hollow particles is 130 nm in physiological pH and it is consistent over a long period. To the best of our knowledge this is the first report on fluorescent magnetic hollow spheres loaded with multiple therapeutic cargoes as well as a magnetic resonance imaging (MRI) contrast agent. The as prepared hollow spheres are biocompatible. The internalization efficiency of the drug loaded particle has been evaluated on folate receptor overexpressed (FR+ve) HeLa, FR-ve HaCat and 3T3 cells. These drug loaded nanospheres exhibit enhanced cytotoxicity as compared to individual drugs. Such a strategy in the simultaneous administration of pemetrexed and platin drugs may open up opportunities for the treatment of lung cancer at its early stage.
具有治疗和诊断功能的治疗诊断型介孔中空二氧化硅纳米球已研制成功。中空介孔二氧化硅纳米球的外表面经过选择性酸功能化处理，用于偶联抗癌药物顺铂、标记分子叶酸（FA）和异硫氰酸罗丹明（RITC），而内部空间则用于包封超顺磁性CoFe2O4纳米颗粒以及疏水性抗癌药物培美曲塞。合成的负载多种药物的中空颗粒在生理pH值下的流体动力学尺寸为130 nm，且在很长一段时间内保持一致。据我们所知，这是关于负载多种治疗性药物以及磁共振成像（MRI）造影剂的荧光磁性中空球的首次报道。所制备的中空球具有生物相容性。已在叶酸受体过表达（FR+ve）的HeLa细胞、FR阴性的HaCaT细胞和3T3细胞上评估了载药颗粒的内化效率。与单一药物相比，这些载药纳米球表现出增强的细胞毒性。这种同时施用培美曲塞和铂类药物的策略可能为早期肺癌的治疗开辟机会。
