Leipold H R, Goldberg R L, Lust G
James A. Baker Institute for Animal Health, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Arthritis Rheum. 1989 Mar;32(3):312-21. doi: 10.1002/anr.1780320313.
Elevated serum levels of keratan sulfate (KS) and hyaluronate (HA) in patients with osteoarthritis (OA) have been reported. We measured KS and HA in dogs to determine if there was an elevation of these serum glycosaminoglycans in a canine model of OA. A single intraarticular injection of 1 mg of chymopapain into a shoulder joint increased serum KS by tenfold, and HA by less than twofold, in 24 hours. Serum KS and HA levels were 3-5-fold higher in dogs younger than 2 months of age than in older dogs. Serum KS and HA concentrations and synovial fluid KS concentrations were unrelated to spontaneous cartilage degeneration in 1-year-old dogs. Higher KS levels in synovial fluid correlated with higher KS levels in serum (r = 0.54, P less than 0.025). The mean KS concentration in sera of older dogs (greater than 3 years old) with OA was 37% higher than that in disease-free controls, but the difference between the groups was not statistically significant. Thus, elevated levels of serum KS and HA do not appear to have clinical significance in this model of OA.
据报道，骨关节炎（OA）患者血清中硫酸角质素（KS）和透明质酸（HA）水平升高。我们对犬类进行了KS和HA的测量，以确定在犬OA模型中这些血清糖胺聚糖是否升高。向肩关节单次关节内注射1毫克木瓜凝乳蛋白酶，24小时内血清KS升高了10倍，HA升高不到2倍。2月龄以下犬的血清KS和HA水平比老年犬高3至5倍。1岁犬的血清KS和HA浓度以及滑液KS浓度与自发性软骨退变无关。滑液中较高的KS水平与血清中较高的KS水平相关（r = 0.54，P < 0.025）。患OA的老年犬（大于3岁）血清中KS的平均浓度比无病对照组高37%，但两组之间的差异无统计学意义。因此，在该OA模型中，血清KS和HA水平升高似乎没有临床意义。
