Strýĉková T, Farbakyová G
Folia Microbiol (Praha). 1976;21(1):21-7. doi: 10.1007/BF02879002.
Lipopolysaccharides (LPS) of Salmonella typhi strains, isolated from carriers and patients suffering from typhoid fever, were characterised according to their biochemical properties, morphological structure and degree of aggregation of complexes. All preparations of LPS, regardless of their origin, were morphologically heterogeneous. Free electrophoresis and immunoelectrophoresis demonstrated that LPS preparations were composed of components possessing different mobilities in electric fields. LPS of bacterial strains isolated from both carriers and patients, split upon reaction in immunoelectrophoresis with specific antiserum 73, rabbit antiserum to Salmonella typhi Vi Bhatnagar and 0-901 split into anodic and cathodic fractions. The anodic fraction reacted similarly as Vi antigen. LPS from Salmonella typhi Ty-2 yielded only the cathodic fraction, typical for O antigen. LPS from strains which were passaged twice in nutritional medium possessed identical properties as LPS from fresh cultures of Salmonella typhi. Electron microscopy revealed that LPS appears as long bands, rods, ellipsoid forms and amorphous material. Contrary to amorphous material, the bands, rods and ellipsoid forms possessed three-layer structure.
从伤寒带菌者和伤寒患者中分离出的伤寒沙门氏菌菌株的脂多糖（LPS），根据其生化特性、形态结构和复合物聚集程度进行了表征。所有LPS制剂，无论其来源如何，在形态上都是异质的。自由电泳和免疫电泳表明，LPS制剂由在电场中具有不同迁移率的成分组成。从带菌者和患者中分离出的细菌菌株的LPS，在与特异性抗血清73（兔抗伤寒沙门氏菌Vi Bhatnagar和0 - 901抗血清）进行免疫电泳反应时会分裂成阳极和阴极部分。阳极部分的反应与Vi抗原相似。伤寒沙门氏菌Ty - 2的LPS只产生阴极部分，这是O抗原的典型特征。在营养培养基中传代两次的菌株的LPS与伤寒沙门氏菌新鲜培养物的LPS具有相同的特性。电子显微镜显示，LPS呈现为长带、杆状、椭圆形和无定形物质。与无定形物质相反，带、杆和椭圆形具有三层结构。
