Branquinho Raquel, Meirinhos-Soares Luís, Carriço João A, Pintado Manuela, Peixe Luísa V
Laboratório de Microbiologia, Departamento de Ciências Biológicas, Faculdade de Farmácia, REQUIMTE, Universidade do Porto, Porto, Portugal.
FEMS Microbiol Ecol. 2014 Dec;90(3):689-98. doi: 10.1111/1574-6941.12426. Epub 2014 Oct 6.
Bacillus pumilus is a Gram-positive bacterium with a wide range of attributed applications, namely as a plant growth promoting rhizobacteria (PGPR), animal, and human probiotic. However, a rare putative role in human diseases has been reported, namely in food poisoning or as anthrax-like cutaneous infectious agent. This species is difficult to distinguish from its closely related species on the basis of phenotypic or biochemical characteristics and 16S rRNA gene sequences. In this study, the phylogenetic analysis of gyrB and rpoB gene sequences of a collection of isolates previously identified as B. pumilus, assigned most of them (93%, 38 of 41 isolates) to B. safensis or to the new recently described B. invictae. Moreover, we extended the previously reported recognized habitats of these species and unveiled a human health or biotechnological relevance (e.g. as implicated in food poisoning or PGPR) for them. Additionally, we demonstrated that both B. safensis and B. invictae species encompass a clonally diverse population, which can justify their great adaptation ability to different niches, with evidence of clonal-host specificity.
短小芽孢杆菌是一种革兰氏阳性菌，具有广泛的应用属性，即作为植物根际促生细菌（PGPR）、动物和人类益生菌。然而，已有报道称其在人类疾病中具有罕见的假定作用，即在食物中毒或作为炭疽样皮肤感染病原体方面。基于表型或生化特征以及16S rRNA基因序列，该物种很难与其密切相关的物种区分开来。在本研究中，对先前鉴定为短小芽孢杆菌的一组分离株的gyrB和rpoB基因序列进行系统发育分析，结果显示其中大部分（93%，41株分离株中的38株）属于阪崎肠杆菌或最近新描述的无敌芽孢杆菌。此外，我们扩展了这些物种先前报道的已知栖息地，并揭示了它们与人类健康或生物技术的相关性（例如与食物中毒或植物根际促生细菌有关）。此外，我们证明阪崎肠杆菌和无敌芽孢杆菌这两个物种都包含克隆多样化的群体，这可以解释它们对不同生态位的强大适应能力，并有克隆-宿主特异性的证据。
