Liu Yang, Lai Qiliang, Du Juan, Shao Zongze
State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2015 Aug;65(8):2769-2773. doi: 10.1099/ijs.0.000336. Epub 2015 May 14.
The aim of this study was to reclarify the taxonomic status of strain Bacillus invictae Bi.FFUP1T by performing comparative analyses with the other four type strains within the Bacillus pumilus group. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains B. invictae Bi.FFUP1T ( = DSMZ 26896T = MCCC 1A07089T), B. altitudinis 41KF2bT ( = DSMZ 21631T = MCCC 1A06452T), B. safensis FO-36bT ( = DSMZ 19292T = MCCC 1A6451T), B. pumilus ATCC 7061T ( = DSMZ 27T = MCCC 1A06453T) and B. xiamenensis HYC-10T ( = MCCC 1A00008T) were, respectively, 82.90 % and 98.10 %, which are greater than the thresholds for bacterial species delineation, suggesting that they should belong to the same species, while the dDDH and ANI values between strain B. invictae DSMZ 26896T and the other three type strains within the B. pumilus group were below the respective thresholds of 70 % and 95 %. Meanwhile, B. invictae DSMZ 26896T and B. altitudinis 41KF2bT shared 98.7 % gyrB gene sequence similarity based on resequencing, whereas strain B. invictae DSMZ 26896T shared low similarities ( < 95 %) with the other three type strains. In addition, in comparison with those from the other three type strains, phenotypic data of B. invictae DSMZ 26896T and B. altitudinis 41KF2bT, including API 20NE, API ZYM, Biolog GN2 and API 50CHB tests, showed slight differences. The data from these combined genotypic and phenotypic analyses suggest that Bacillus invictae Branquinho et al. 2014 should be regarded as a later heterotypic synonym of Bacillus altitudinisShivaji et al. 2006.
本研究的目的是通过与短小芽孢杆菌组内的其他四株模式菌株进行比较分析，重新明确无敌芽孢杆菌菌株Bi.FFUP1T的分类地位。无敌芽孢杆菌菌株Bi.FFUP1T（=DSMZ 26896T =MCCC 1A07089T）、高地芽孢杆菌41KF2bT（=DSMZ 21631T =MCCC 1A06452T）、安全岛芽孢杆菌FO-36bT（=DSMZ 19292T =MCCC 1A6451T）、短小芽孢杆菌ATCC 7061T（=DSMZ 27T =MCCC 1A06453T）和厦门芽孢杆菌HYC-10T（=MCCC 1A00008T）之间的数字DNA-DNA杂交（dDDH）值和平均核苷酸同一性（ANI）值分别为82.90%和98.10%，均高于细菌物种划分的阈值，表明它们应属于同一物种，而无敌芽孢杆菌DSMZ 26896T菌株与短小芽孢杆菌组内其他三株模式菌株之间的dDDH值和ANI值均低于各自70%和95%的阈值。同时，基于重测序，无敌芽孢杆菌DSMZ 26896T和高地芽孢杆菌41KF2bT的gyrB基因序列相似性为98.7%，而无敌芽孢杆菌DSMZ 26896T菌株与其他三株模式菌株的相似性较低（<95%）。此外，与其他三株模式菌株相比，无敌芽孢杆菌DSMZ 26896T和高地芽孢杆菌41KF2bT的表型数据，包括API 20NE、API ZYM、Biolog GN2和API 50CHB测试，显示出细微差异。这些综合的基因型和表型分析数据表明，2014年的无敌芽孢杆菌Branquinho等人应被视为2006年的高地芽孢杆菌Shivaji等人的晚出异名。
