桃仁提取物中分离得到的苦杏仁苷可诱导 HepG2 和 C2C12 细胞系中卵泡抑素的表达。
Amygdalin isolated from Semen Persicae (Tao Ren) extracts induces the expression of follistatin in HepG2 and C2C12 cell lines.
机构信息
School of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 10 Sassoon Road, Pokfulam, Hong Kong, China.
Department of Chemistry, The University of Hong Kong, Hong Kong, China.
出版信息
Chin Med. 2014 Sep 16;9:23. doi: 10.1186/1749-8546-9-23. eCollection 2014.
BACKGROUND
The Chinese medicine formulation ISF-1 (also known as Bu-Yang-Huan-Wu-Tang) for post-stroke rehabilitation could increase the expression of growth-regulating protein follistatin by approximately 4-fold. This study aims to identify the active compounds of ISF-1 for the induction of follistatin expression.
METHODS
Active compounds in ISF-1 responsible for induction of follistatin were identified by a bioactivity-guided fractionation procedure involving liquid-liquid extraction, HPLC separation and RT-PCR detection. The aqueous extracts of seven ISF-1 ingredients including Semen Persicae (Tao Ren) and the S. Persicae-derived fractions were assayed for the induction of follistatin mRNA expression in human hepatocarcinoma HepG2 cells by RT-PCR. The concentrations of isolated compounds were proportionally normalized to the reported IC50 concentration (5.8 mg/mL) of the formulation ISF-1 in HepG2. The active fractions were characterized by reverse-phase HPLC on a C18 column and identified by mass spectrometry.
RESULTS
Three ingredients of ISF-1, namely S. Persicae (Tao Ren), Pheretima (Di Long), and Flos Carthami (Hong Hua), induced the expression of follistatin mRNA. Among these, the ingredient S. Persicae were the most active, and amygdalin from S. Persicae extract was identified as a novel follistatin inducer. Amygdalin stimulated the growth of skeletal muscle cell line C2C12 cells in a concentration-dependent manner.
CONCLUSIONS
Amygdalin isolated from S. Persicae extract in ISF-1 through a bioactivity-guided fractionation procedure induced the expression of follistatin in HepG2 and C2C12 cell lines.
背景
用于中风后康复的中药配方 ISF-1（也称为补阳还五汤）可以使生长调节蛋白卵泡抑素的表达增加约 4 倍。本研究旨在确定 ISF-1 中诱导卵泡抑素表达的活性化合物。
方法
通过涉及液 - 液萃取、HPLC 分离和 RT-PCR 检测的基于生物活性的分级程序，鉴定 ISF-1 中负责诱导卵泡抑素表达的活性化合物。七种 ISF-1 成分（包括桃仁）的水提取物及其桃仁衍生的部分，通过 RT-PCR 检测在人肝癌 HepG2 细胞中诱导卵泡抑素 mRNA 表达的能力。分离化合物的浓度按比例归一化为配方 ISF-1 在 HepG2 中的报告 IC50 浓度（5.8mg/mL）。通过 C18 柱反相 HPLC 和质谱对活性部分进行表征。
结果
ISF-1 的三种成分，即桃仁、地龙和红花，诱导了卵泡抑素 mRNA 的表达。其中，桃仁成分最活跃，桃仁提取物中的苦杏仁苷被鉴定为一种新的卵泡抑素诱导剂。苦杏仁苷以浓度依赖的方式刺激骨骼肌细胞系 C2C12 细胞的生长。
结论
通过基于生物活性的分级程序从 ISF-1 中的桃仁提取物中分离出的苦杏仁苷诱导 HepG2 和 C2C12 细胞系中卵泡抑素的表达。
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