Melnikova Nataliya V, Kudryavtseva Anna V, Zelenin Alexander V, Lakunina Valentina A, Yurkevich Olga Yu, Speranskaya Anna S, Dmitriev Alexey A, Krinitsina Anastasia A, Belenikin Maxim S, Uroshlev Leonid A, Snezhkina Anastasiya V, Sadritdinova Asiya F, Koroban Nadezda V, Amosova Alexandra V, Samatadze Tatiana E, Guzenko Elena V, Lemesh Valentina A, Savilova Anastasya M, Rachinskaia Olga A, Kishlyan Natalya V, Rozhmina Tatiana A, Bolsheva Nadezhda L, Muravenko Olga V
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia ; Department of Higher Plants, Lomonosov Moscow State University, Moscow 119991, Russia.
Biomed Res Int. 2014;2014:231589. doi: 10.1155/2014/231589. Epub 2014 Aug 27.
SSAP method was used to study the genetic diversity of 22 Linum species from sections Linum, Adenolinum, Dasylinum, Stellerolinum, and 46 flax cultivars. All the studied flax varieties were distinguished using SSAP for retrotransposons FL9 and FL11. Thus, the validity of SSAP method was demonstrated for flax marking, identification of accessions in genebank collections, and control during propagation of flax varieties. Polymorphism of Fl1a, Fl1b, and Cassandra insertions were very low in flax varieties, but these retrotransposons were successfully used for the investigation of Linum species. Species clusterization based on SSAP markers was in concordance with their taxonomic division into sections Dasylinum, Stellerolinum, Adenolinum, and Linum. All species of sect. Adenolinum clustered apart from species of sect. Linum. The data confirmed the accuracy of the separation in these sections. Members of section Linum are not as closely related as members of other sections, so taxonomic revision of this section is desirable. L. usitatissimum accessions genetically distant from modern flax cultivars were revealed in our work. These accessions are of utmost interest for flax breeding and introduction of new useful traits into flax cultivars. The chromosome localization of Cassandra retrotransposon in Linum species was determined.
采用SSAP方法研究了亚麻组、腺亚麻组、粗齿亚麻组、矮亚麻组的22个亚麻属物种以及46个亚麻栽培品种的遗传多样性。利用SSAP技术针对反转录转座子FL9和FL11对所有研究的亚麻品种进行了区分。因此，证明了SSAP方法在亚麻标记、基因库收集品中种质鉴定以及亚麻品种繁殖过程中的监控方面的有效性。亚麻品种中Fl1a、Fl1b和卡珊卓插入序列的多态性非常低，但这些反转录转座子成功用于亚麻属物种的研究。基于SSAP标记的物种聚类与其分类学上分为粗齿亚麻组、矮亚麻组、腺亚麻组和亚麻组的划分一致。腺亚麻组的所有物种都与亚麻组的物种聚类分开。数据证实了这些组划分的准确性。亚麻组的成员之间的亲缘关系不像其他组的成员那样密切，因此该组的分类学修订是可取的。在我们的研究中发现了与现代亚麻栽培品种遗传距离较远的亚麻栽培品种。这些种质对于亚麻育种以及将新的有用性状引入亚麻栽培品种具有极大的意义。确定了卡珊卓反转录转座子在亚麻属物种中的染色体定位。
