Wu Tao, Xie Guoxiang, Ni Yan, Liu Tao, Yang Ming, Wei Huafeng, Jia Wei, Ji Guang
Center of Chinese Medical Therapy and Systems Biology, Shanghai University of Traditional Chinese Medicine , 1200 Cailun Road, Shanghai 201203, China.
J Proteome Res. 2015 Jan 2;14(1):447-56. doi: 10.1021/pr500825y. Epub 2014 Oct 6.
A number of metabolic conditions, including hypoglycemia, high blood pressure (HBP), dyslipidemia, nerve damage and amputation, and vision problems, occur as a result of uncontrolled blood glucose levels over a prolonged period of time. The different components of diabetic complications are not independent but rather interdependent of each other, rendering the disease difficult to diagnose and control. The underlying pathogenesis of those components cannot be easily elucidated because of the heterogeneous, polygenic, and multifactorial nature of the disease. Metabonomics offers a snapshot of distinct biochemical variations that may reflect the unique metabolic phenotype under pathophysiological conditions. Here we report a mass-spectrometry-based metabonomic study designed to identify the distinct metabolic changes associated with several complications of type 2 diabetes mellitus (T2DM). The 292 patients recruited in the study were divided into five groups, including T2DM with HBP, T2DM with nonalcoholic fatty liver disease (NAFLD), T2DM with HBP and NAFLD, T2DM with HBP and coronary heart disease (CHD), and T2DM with HBP, NAFLD, and CHD. Serum differential metabolites were identified in each group of T2DM complication, mainly involving bile acid, fatty acid, amino acid, lipid, carbohydrate, steroids metabolism, and tricarboxylic acids cycle. These broad-spectrum metabolic changes emphasize the complex abnormalities present among these complications with elevated blood glucose levels, providing a novel strategy for stratifying patients with T2DM complications using blood-based metabolite markers.
长期血糖水平失控会引发多种代谢状况，包括低血糖、高血压（HBP）、血脂异常、神经损伤与截肢以及视力问题。糖尿病并发症的不同组成部分并非相互独立，而是相互依存，这使得该疾病难以诊断和控制。由于该疾病具有异质性、多基因性和多因素性，这些组成部分的潜在发病机制难以轻易阐明。代谢组学提供了不同生化变化的概况，这些变化可能反映病理生理条件下独特的代谢表型。在此，我们报告一项基于质谱的代谢组学研究，旨在识别与2型糖尿病（T2DM）几种并发症相关的独特代谢变化。该研究招募的292名患者被分为五组，包括患有HBP的T2DM、患有非酒精性脂肪性肝病（NAFLD）的T2DM、患有HBP和NAFLD的T2DM、患有HBP和冠心病（CHD）的T2DM以及患有HBP、NAFLD和CHD的T2DM。在每组T2DM并发症中均鉴定出血清差异代谢物，主要涉及胆汁酸、脂肪酸、氨基酸、脂质、碳水化合物、类固醇代谢以及三羧酸循环。这些广谱代谢变化突显了血糖水平升高时这些并发症中存在的复杂异常情况，为使用基于血液的代谢物标记物对T2DM并发症患者进行分层提供了一种新策略。
