Marinho Catarina, Igrejas Gilberto, Gonçalves Alexandre, Silva Nuno, Santos Tiago, Monteiro Ricardo, Gonçalves David, Rodrigues Tiago, Poeta Patrícia
Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro, Apartado 1013, 5001-801 Vila Real, Portugal; Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Apartado 1013, 5001-801 Vila Real, Portugal; Centre of Studies of Animal and Veterinary Sciences, Apartado 1013, 5001-801 Vila Real, Portugal; Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, Apartado 1013, 5001-801 Vila Real, Portugal.
Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro, Apartado 1013, 5001-801 Vila Real, Portugal; Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Apartado 1013, 5001-801 Vila Real, Portugal.
Anaerobe. 2014 Dec;30:116-9. doi: 10.1016/j.anaerobe.2014.09.009. Epub 2014 Sep 20.
Antibiotic resistance in bacteria is an increasing problem that is not only constrained to the clinical setting but also to other environments that can lodge antibiotic resistant bacteria and therefore they may serve as reservoirs of genetic determinants of antibiotic resistance. One hundred and thirty-six faecal samples from European wild rabbits (Oryctolagus cuniculus algirus) were collected on São Jorge Island in Azores Archipelago, and analysed for Escherichia coli isolates. Seventy-seven isolates (56.6%) were recovered and studied for antimicrobial resistance, one isolate per positive sample. Thirteen (16.9%), 19 (24.7%), 25 (32.4%) and 20 (26%) isolates were ascribed to A, B1, B2 and D phylogenetic groups, respectively, by specific primer polymerase chain reaction. Different E. coli isolates were found to be resistant to ampicillin (16.9%), tetracycline (1.3%), streptomycin (42.9%), sulfamethoxazole-trimethoprim (1.3%), amikacin (1.3%), tobramycin (2.6%) and nalidixic acid (1.3%). Additionally, the blaTEM, tetA, strA/strB, aadA, sul1, intI, intI2 and qacEΔ+sul1 genes were found in most resistant isolates. This study showed that E. coli from the intestinal tract of wild rabbits from Azores Archipelago are resistant to widely prescribed antibiotics in medicine and they constitute a reservoir of antimicrobial resistant genes, which may play a significant role in the spread of antimicrobial resistance. Therefore, antibiotic resistant E. coli from Azorean wild rabbits may represent an ecological and public health problem.
细菌中的抗生素耐药性问题日益严重，这不仅局限于临床环境，还涉及其他可能存在抗生素耐药菌的环境，因此这些环境可能成为抗生素耐药性基因决定因素的储存库。在亚速尔群岛的圣若热岛采集了136份欧洲野兔（穴兔阿尔及利亚亚种）的粪便样本，并对其中的大肠杆菌分离株进行了分析。共分离出77株（56.6%）并对其进行了抗菌药物耐药性研究，每个阳性样本分离出一株。通过特异性引物聚合酶链反应，分别有13株（16.9%）、19株（24.7%）、25株（32.4%）和20株（26%）分离株被归为A、B1、B2和D系统发育组。发现不同的大肠杆菌分离株对氨苄西林（16.9%）、四环素（1.3%）、链霉素（42.9%）、磺胺甲恶唑-甲氧苄啶（1.3%）、阿米卡星（1.3%）、妥布霉素（2.6%）和萘啶酸（1.3%）耐药。此外，在大多数耐药分离株中发现了blaTEM、tetA、strA/strB、aadA、sul1、intI、intI2和qacEΔ+sul1基因。这项研究表明，亚速尔群岛野兔肠道中的大肠杆菌对医学上广泛使用的抗生素耐药，它们构成了抗菌药物耐药基因的储存库，这可能在抗菌药物耐药性传播中发挥重要作用。因此，亚速尔群岛野兔携带的抗生素耐药性大肠杆菌可能代表了一个生态和公共卫生问题。
