Biomedical Health Sciences, University of Health Sciences of Porto Alegre-UFCSPA, Rio Grande do Sul, Brazil.
Biomedicine, Institute of Health Sciences, University Feevale, Rio Grande do Sul, Brazil.
Nutr Res. 2014 Sep;34(9):742-8. doi: 10.1016/j.nutres.2014.08.009. Epub 2014 Aug 28.
Because of their numerous roles in several biological processes, zinc and selenium are the most commonly studied micronutrients in the elderly. Therefore, we hypothesized that the polymorphisms in the genes that are responsible for the transport of zinc and selenium may have a genotype-dependent effect on the serum concentration of these micronutrients. The objective of this study was to determine the effects of solute carrier family 30 member 3 (SLC30A3) and 15-kd selenoprotein (SEP15) polymorphisms on zinc and selenium concentrations, respectively, in the serum. This cross-sectional study included 110 individuals who were aged 50 years or older. Serum micronutrient concentrations were determined by flame atomic absorption spectrophotometry (for zinc) and by atomic absorption spectrophotometry with a graphite furnace (for selenium). The single-nucleotide polymorphisms, rs73924411 and rs11126936 of the SLC30A3 gene and rs5859, rs5854, and rs561104 of the SEP15 gene, were examined by real-time polymerase chain reaction. Regarding rs11126936, the serum zinc concentration was lower in CC homozygotes (0.75 ± 0.31 mg/L) than in A carriers (0.89 ± 0.28 mg/L, P = .016). Concerning rs561104, the serum selenium concentration was higher in CC homozygotes (5.65 ± 1.11 μg/dL) compared with T carriers (4.88 ± 1.25 μg/dL, P = .044). Our results demonstrate the influence of SLC30A3 and SEP15 gene polymorphisms on the serum concentrations of zinc and selenium, respectively. The effects of these associations should be further investigated to help elucidate the modes of action of trace elements and to identify biomarkers, which could ultimately define the optimal intake of these micronutrients at the molecular level. More research must be performed before the roles of these polymorphisms in the serum concentrations of zinc and selenium can be fully understood.
由于锌和硒在多种生物过程中发挥着重要作用，因此它们是老年人中研究最多的微量营养素。因此，我们假设负责锌和硒运输的基因中的多态性可能对这些微量营养素的血清浓度具有基因型依赖性影响。本研究的目的是确定溶质载体家族 30 成员 3（SLC30A3）和 15-kd 硒蛋白（SEP15）基因多态性对血清中锌和硒浓度的分别影响。这项横断面研究纳入了 110 名年龄在 50 岁或以上的个体。通过火焰原子吸收分光光度法（用于锌）和石墨炉原子吸收分光光度法（用于硒）测定血清微量营养素浓度。通过实时聚合酶链反应检测 SLC30A3 基因的 rs73924411 和 rs11126936 单核苷酸多态性，以及 SEP15 基因的 rs5859、rs5854 和 rs561104。关于 rs11126936，CC 纯合子（0.75 ± 0.31 mg/L）的血清锌浓度低于 A 携带者（0.89 ± 0.28 mg/L，P =.016）。关于 rs561104，CC 纯合子（5.65 ± 1.11 μg/dL）的血清硒浓度高于 T 携带者（4.88 ± 1.25 μg/dL，P =.044）。我们的结果表明 SLC30A3 和 SEP15 基因多态性分别对锌和硒的血清浓度有影响。这些关联的影响应进一步研究，以帮助阐明微量元素的作用模式并确定生物标志物，最终在分子水平上确定这些微量营养素的最佳摄入量。在充分了解这些多态性在锌和硒血清浓度中的作用之前，还需要进行更多的研究。
