Patra Jayanta Kumar, Das Swagat Kumar, Thatoi Hrudayanath
Department of Biotechnology, College of Engineering and Technology, Biju Patnaik University of Technology, Bhubaneswar, 751003, India.
Chin J Integr Med. 2015 Apr;21(4):274-85. doi: 10.1007/s11655-014-1854-y. Epub 2014 Sep 24.
To test the antioxidant, antidiabetic, anticancer and antibacterial activities along with phytochemicals of Sonneratia apetala Buch.-Ham.
The antibacterial activity was determined by agar well diffusion method. The antioxidant activity was determined by standard assay. The antidiabetic activity was evaluated by α-glucosidase inhibition assay and in vivo anticancer property was determined against Ehrlich ascites carcinoma (EAC) cells in Swiss Albino mice. Further partial characterization of the methanol extracts was carried out by thin layer chromatography, high performance liquid chromatography, (1)H nuclear magnetic resonance spectroscopy and Fourier transform-infra red spectrum spectral analysis.
Four solvent extracts (acetone, ethanol, methanol and aqueous) of leaf and bark possess strong antioxidant properties. In vivo anticancer activity of methanol extract leaf indicated positive activity showing 34% inhibition against EAC cells in Swiss Albino mice. All extracts exhibited α-glucosidase inhibitory activity in a dose-dependent manner indicating presence of promising antidiabetic properties. The extracts possess strong antibacterial activity against the selected pathogenic bacteria (minimal inhibitory concentration ranging from 1.25-5.00 mg/mL). The partial characterization of the methanol extracts of leaf and bark revealed the presence of phenolics as the lead compound responsible for studied bioactivities of the plant extracts.
Sonneratia apetala extracts have potent antibacterial, antioxidant, antidiabetic and anticancer properties which can be further exploited for its pharmaceutical applications.
测试无瓣海桑的抗氧化、抗糖尿病、抗癌和抗菌活性以及植物化学成分。
采用琼脂扩散法测定抗菌活性。通过标准测定法测定抗氧化活性。通过α-葡萄糖苷酶抑制试验评估抗糖尿病活性，并在瑞士白化小鼠中针对艾氏腹水癌（EAC）细胞测定体内抗癌特性。通过薄层色谱、高效液相色谱、（1）H核磁共振光谱和傅里叶变换红外光谱分析对甲醇提取物进行进一步的部分表征。
叶和树皮的四种溶剂提取物（丙酮、乙醇、甲醇和水）具有很强的抗氧化性能。甲醇提取物叶的体内抗癌活性显示出阳性活性，对瑞士白化小鼠的EAC细胞有34%的抑制作用。所有提取物均以剂量依赖方式表现出α-葡萄糖苷酶抑制活性，表明具有良好的抗糖尿病特性。提取物对所选病原菌具有很强的抗菌活性（最低抑菌浓度范围为1.25 - 5.00mg/mL）。叶和树皮甲醇提取物的部分表征显示，酚类物质作为主要化合物存在，其对植物提取物的生物活性起作用。
无瓣海桑提取物具有强大的抗菌、抗氧化、抗糖尿病和抗癌特性，可进一步用于医药应用。
