高危急诊科患者中 Xpert Flu 快速 PCR 检测的评估。
Evaluation of the Xpert Flu rapid PCR assay in high-risk emergency department patients.
机构信息
Department of Emergency Medicine, Johns Hopkins University, Baltimore, Maryland, USA
Department of Pathology, Johns Hopkins University, Baltimore, Maryland, USA.
出版信息
J Clin Microbiol. 2014 Dec;52(12):4353-5. doi: 10.1128/JCM.02343-14. Epub 2014 Sep 24.
Abstract
We prospectively evaluated the performance of Cepheid's GeneXpert Xpert Flu assay in a target population of 281 adults presenting to the emergency department with an acute respiratory illness who met Centers for Disease Control and Prevention (CDC) criteria for recommended antiviral treatment. Compared with the Prodesse ProFlu+ assay, Xpert Flu had an overall sensitivity of 95.3% and specificity of 99.2%.
摘要
我们前瞻性地评估了 Cepheid 公司的 GeneXpert Xpert Flu 检测在符合美国疾病控制与预防中心（CDC）推荐抗病毒治疗标准的 281 例急性呼吸道疾病就诊于急诊科的成年目标人群中的表现。与 Prodesse ProFlu+ 检测相比，Xpert Flu 的总敏感性为 95.3%，特异性为 99.2%。
相似文献
1
Evaluation of the Xpert Flu rapid PCR assay in high-risk emergency department patients.高危急诊科患者中 Xpert Flu 快速 PCR 检测的评估。
J Clin Microbiol. 2014 Dec;52(12):4353-5. doi: 10.1128/JCM.02343-14. Epub 2014 Sep 24.
2
A novel PCR-based point-of-care method facilitates rapid, efficient, and sensitive diagnosis of influenza virus infection.一种新型基于 PCR 的即时检测方法可实现流感病毒感染的快速、高效和敏感诊断。
Clin Microbiol Infect. 2019 Aug;25(8):1032-1037. doi: 10.1016/j.cmi.2018.12.017. Epub 2018 Dec 22.
3
Diagnostic performances of the Xpert Flu PCR test and the OSOM immunochromatographic rapid test for influenza A and B virus among adult patients in the Emergency Department.Xpert Flu PCR 检测与 OSOM 免疫层析快速检测法在急诊科成年患者中用于甲型和乙型流感病毒的诊断性能。
J Clin Virol. 2018 Feb-Mar;99-100:5-9. doi: 10.1016/j.jcv.2017.12.005. Epub 2017 Dec 13.
4
Prospective and Retrospective Evaluation of the Performance of the FDA-Approved Cepheid Xpert Flu/RSV XC Assay.对美国食品药品监督管理局（FDA）批准的赛沛Xpert流感/呼吸道合胞病毒（RSV）XC检测性能的前瞻性和回顾性评估。
Lab Med. 2017 Nov 8;48(4):e53-e56. doi: 10.1093/labmed/lmx038.
5
Performance Characteristics of Xpert Flu/RSV XC Assay.Xpert Flu/RSV XC检测的性能特征
J Clin Microbiol. 2015 Aug;53(8):2720-1. doi: 10.1128/JCM.00972-15. Epub 2015 May 27.
6
Accurate PCR Detection of Influenza A/B and Respiratory Syncytial Viruses by Use of Cepheid Xpert Flu+RSV Xpress Assay in Point-of-Care Settings: Comparison to Prodesse ProFlu.利用 Cepheid Xpert Flu+RSV Xpress 检测试剂盒在即时检测点对流感 A/B 和呼吸道合胞病毒的精确 PCR 检测：与 Prodesse ProFlu 的比较。
J Clin Microbiol. 2018 Jan 24;56(2). doi: 10.1128/JCM.01237-17. Print 2018 Feb.
7
Xpert Flu as a rapid diagnostic test for respiratory tract viral infection: evaluation and implementation as a 24/7 service.Xpert Flu 作为一种呼吸道病毒感染的快速诊断检测方法：作为一项 24/7 服务的评估与实施。
Infect Dis (Lond). 2018 Feb;50(2):140-144. doi: 10.1080/23744235.2017.1380841. Epub 2017 Sep 26.
8
Verification of Cepheid Xpert Xpress Flu/RSV assay for use with gargle samples, sputa and endotracheal secretions.验证赛沛Xpert Xpress流感/呼吸道合胞病毒检测法用于漱口水样本、痰液和气管内分泌物检测的效果。
J Hosp Infect. 2019 Jan;101(1):114-115. doi: 10.1016/j.jhin.2018.07.016. Epub 2018 Jul 17.
9
Evaluation of the Cepheid Xpert Flu Assay for rapid identification and differentiation of influenza A, influenza A 2009 H1N1, and influenza B viruses.评估赛沛 Xpert 流感检测法用于快速鉴定和区分甲型流感、甲型 H1N1 流感和乙型流感病毒。
J Clin Microbiol. 2012 May;50(5):1704-10. doi: 10.1128/JCM.06520-11. Epub 2012 Feb 29.
10
Rapid diagnosis of influenza: an evaluation of two commercially available RT-PCR assays.快速诊断流感：两种市售 RT-PCR 检测方法的评估。
J Infect. 2012 Jul;65(1):60-3. doi: 10.1016/j.jinf.2012.04.003. Epub 2012 Apr 17.
引用本文的文献
1
An open source, PCR based, point-of-care testing platform.一个基于聚合酶链式反应（PCR）的开源即时检测平台。
Sci Rep. 2025 Apr 8;15(1):12025. doi: 10.1038/s41598-025-95639-x.
2
How Significant Are Xpert Xpress SARS-CoV-2 Test Findings When Only an N2 Gene Is Detected?仅检测到N2基因时，Xpert Xpress SARS-CoV-2检测结果的意义有多大？
Diagnostics (Basel). 2022 Sep 2;12(9):2133. doi: 10.3390/diagnostics12092133.
3
Cloud-Based Influenza Surveillance System in Emergency Departments Using Molecular-Based Testing: Advances and Challenges.基于云的使用分子检测的急诊科流感监测系统：进展与挑战。
West J Emerg Med. 2022 Feb 14;23(2):115-123. doi: 10.5811/westjem.2021.9.52741.
4
COVID-19 Pandemic and Upcoming Influenza Season-Does an Expert's Computed Tomography Assessment Differentially Identify COVID-19, Influenza and Pneumonias of Other Origin?新冠疫情与即将到来的流感季——专家的计算机断层扫描评估能否鉴别新冠病毒感染、流感及其他原因引起的肺炎？
J Clin Med. 2020 Dec 28;10(1):84. doi: 10.3390/jcm10010084.
5
Diagnostic accuracy of a rapid RT-PCR assay for point-of-care detection of influenza A/B virus at emergency department admission: A prospective evaluation during the 2017/2018 influenza season.在急诊入院时即时检测流感 A/B 病毒的快速 RT-PCR 检测方法的诊断准确性：2017/2018 流感季的前瞻性评估。
PLoS One. 2019 May 7;14(5):e0216308. doi: 10.1371/journal.pone.0216308. eCollection 2019.
6
Laboratory Diagnosis of Respiratory Tract Infections in Children - the State of the Art.儿童呼吸道感染的实验室诊断——最新技术水平
Front Microbiol. 2018 Oct 18;9:2478. doi: 10.3389/fmicb.2018.02478. eCollection 2018.
7
Necessity to critically review the automatic results of the Xpert Flu assay.有必要严格审查Xpert流感检测的自动结果。
Diagn Microbiol Infect Dis. 2017 May;88(1):26-30. doi: 10.1016/j.diagmicrobio.2017.01.015. Epub 2017 Feb 2.
8
Diagnosing Viral and Atypical Pathogens in the Setting of Community-Acquired Pneumonia.社区获得性肺炎中病毒及非典型病原体的诊断
Clin Chest Med. 2017 Mar;38(1):21-28. doi: 10.1016/j.ccm.2016.11.004. Epub 2016 Dec 21.
9
[Rapid diagnostic test for respiratory infections].[呼吸道感染的快速诊断检测]
Enferm Infecc Microbiol Clin. 2017 Feb;35(2):108-115. doi: 10.1016/j.eimc.2016.11.007. Epub 2017 Jan 3.
10
Rapid diagnostic methods for acute viral respiratory infections.急性病毒性呼吸道感染的快速诊断方法
Enferm Infecc Microbiol Clin. 2016 Jun-Jul;34(6):329-30. doi: 10.1016/j.eimc.2016.04.004. Epub 2016 May 5.
本文引用的文献
1
Clinical and virologic factors associated with reduced sensitivity of rapid influenza diagnostic tests in hospitalized elderly patients and young children.与住院老年患者和幼童中快速流感诊断检测敏感性降低相关的临床和病毒学因素。
J Clin Microbiol. 2014 Feb;52(2):497-501. doi: 10.1128/JCM.02316-13. Epub 2013 Nov 27.
2
Clinical application of a molecular method based on real time RT-PCR for detection of influenza A(H1N1)v virus.一种基于实时逆转录聚合酶链反应的分子方法在甲型H1N1流感病毒检测中的临床应用
New Microbiol. 2013 Oct;36(4):405-8. Epub 2013 Oct 1.
3
Improved sensitivity of the novel Xpert flu test for detection of influenza B virus.新型Xpert流感检测法检测乙型流感病毒的灵敏度提高。
J Clin Microbiol. 2013 Dec;51(12):4277-8. doi: 10.1128/JCM.02125-13. Epub 2013 Sep 25.
4
Impact of neuraminidase inhibitor treatment on outcomes of public health importance during the 2009-2010 influenza A(H1N1) pandemic: a systematic review and meta-analysis in hospitalized patients.2009-2010 年甲型 H1N1 流感大流行期间神经氨酸酶抑制剂治疗对具有公共卫生重要性的结局的影响：一项对住院患者的系统评价和荟萃分析。
J Infect Dis. 2013 Feb 15;207(4):553-63. doi: 10.1093/infdis/jis726. Epub 2012 Nov 29.
5
Comparison of Xpert Flu rapid nucleic acid testing with rapid antigen testing for the diagnosis of influenza A and B.Xpert Flu 快速核酸检测与快速抗原检测在甲型和乙型流感诊断中的比较。
J Virol Methods. 2012 Dec;186(1-2):137-40. doi: 10.1016/j.jviromet.2012.07.023. Epub 2012 Jul 25.
6
Evaluation of the Cepheid Xpert Flu Assay for rapid identification and differentiation of influenza A, influenza A 2009 H1N1, and influenza B viruses.评估赛沛 Xpert 流感检测法用于快速鉴定和区分甲型流感、甲型 H1N1 流感和乙型流感病毒。
J Clin Microbiol. 2012 May;50(5):1704-10. doi: 10.1128/JCM.06520-11. Epub 2012 Feb 29.
7
Antivirals for treatment of influenza: a systematic review and meta-analysis of observational studies.抗流感病毒药物治疗：观察性研究的系统评价和荟萃分析。
Ann Intern Med. 2012 Apr 3;156(7):512-24. doi: 10.7326/0003-4819-156-7-201204030-00411. Epub 2012 Feb 27.
8
Evaluation of the Xpert Flu test and comparison with in-house real-time RT-PCR assays for detection of influenza virus from 2008 to 2011 in Marseille, France.2008 年至 2011 年法国马赛地区 Xpert Flu 检测与实时 RT-PCR 检测流感病毒的比较评估。
Clin Microbiol Infect. 2012 Apr;18(4):E81-3. doi: 10.1111/j.1469-0691.2012.03792.x. Epub 2012 Feb 23.
9
Retrospective and prospective verification of the Cepheid Xpert influenza virus assay.回顾性和前瞻性验证赛沛 Xpert 流感病毒检测。
J Clin Microbiol. 2011 Sep;49(9):3368-9. doi: 10.1128/JCM.01162-11. Epub 2011 Jul 20.
10
Evaluation of the Xpert Flu A Panel nucleic acid amplification-based point-of-care test for influenza A virus detection and pandemic H1 subtyping.评估 Xpert Flu A 试剂盒基于核酸扩增的即时检测技术用于甲型流感病毒检测和大流行 H1 亚型鉴定。
J Clin Virol. 2010 Oct;49(2):85-9. doi: 10.1016/j.jcv.2010.07.005. Epub 2010 Jul 31.
Zotero 插件