Xpert Flu 快速核酸检测与快速抗原检测在甲型和乙型流感诊断中的比较。
Comparison of Xpert Flu rapid nucleic acid testing with rapid antigen testing for the diagnosis of influenza A and B.
机构信息
Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
出版信息
J Virol Methods. 2012 Dec;186(1-2):137-40. doi: 10.1016/j.jviromet.2012.07.023. Epub 2012 Jul 25.
Influenza infections are associated with thousands of hospital admissions and deaths each year. Rapid detection of influenza is important for prompt initiation of antiviral therapy and appropriate patient triage. In this study the Cepheid Xpert Flu assay was compared with two rapid antigen tests, BinaxNOW Influenza A & B and BD Directigen EZ Flu A+B, as well as direct fluorescent antibody testing for the rapid detection of influenza A and B. Using real-time, hydrolysis probe-based, reverse transcriptase PCR as the reference method, influenza A sensitivity was 97.3% for Xpert Flu, 95.9% for direct fluorescent antibody testing, 62.2% for BinaxNOW, and 71.6% for BD Directigen. Influenza B sensitivity was 100% for Xpert Flu and direct fluorescent antibody testing, 54.5% for BinaxNOW, and 48.5% for BD Directigen. Specificity for influenza A was 100% for Xpert Flu, BinaxNOW, and BD Directigen, and 99.2% for direct fluorescent antibody testing. All methods demonstrated 100% specificity for influenza B. These findings support the use of the Xpert Flu assay in settings requiring urgent diagnosis of influenza A and B.
流感感染每年导致数千人住院和死亡。快速检测流感对于及时启动抗病毒治疗和适当的患者分诊非常重要。在这项研究中，Cepheid Xpert Flu 检测与两种快速抗原检测（BinaxNOW 流感 A&B 和 BD Directigen EZ Flu A+B）以及直接荧光抗体检测进行了比较，用于快速检测流感 A 和 B。使用实时、基于水解探针的逆转录聚合酶链反应作为参考方法，Xpert Flu 对流感 A 的敏感性为 97.3%，直接荧光抗体检测为 95.9%，BinaxNOW 为 62.2%，BD Directigen 为 71.6%。Xpert Flu 和直接荧光抗体检测对流感 B 的敏感性为 100%，BinaxNOW 为 54.5%，BD Directigen 为 48.5%。Xpert Flu、BinaxNOW 和 BD Directigen 对流感 A 的特异性为 100%，直接荧光抗体检测为 99.2%。所有方法对流感 B 的特异性均为 100%。这些发现支持在需要紧急诊断流感 A 和 B 的情况下使用 Xpert Flu 检测。
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