Hang Hui, Yuan Songtao, Yang Qin, Yuan Dongqing, Liu Qinghuai
Department of ophthalmology, The First Affiliated Hospital of Nanjing Medical University.
Mol Vis. 2014 Aug 4;20:1137-45. eCollection 2014.
The aim of this study was to assess the roles of plasma cytokines in diabetic retinopathy (DR) and their relationship with the severity of DR.
This study included 59 diabetic patients and 19 non-diabetic controls. The plasma concentrations of endothelial growth factor (EGF), eotaxin, fibroblast growth factor 2 (FGF-2), Flt-3 ligand (Flt-3L), fractalkine, granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), growth-related oncogene (GRO), interferon (IFN)-α2, IFN-γ, interleukin (IL)-1α, IL-1β, IL-1Ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17, IFN-inducible protein-10 (IP-10), monocyte chemoattractant protein (MCP)-1, MCP-3, macrophage-derived cytokine (MDC), macrophage inflammatory protein (MIP)-1α, MIP-1β, sCD40L, sIL-2Rα, transforming growth factor (TGF)-α, tumor necrosis factor (TNF)- α, TNF-β, and VEGF were measured with Luminex multiplex bead immunoassay. The levels of these cytokines were investigated according to the DR stage.
The plasma level of ten cytokines-MCP-1, IL-6, IL-7, IL-9, IL-13, IL-15, IL-17, sCD40L, sIL-2Rα and TNF-β-increased significantly in the diabetic group compared to the controls. The Flt-3L, IL-1Ra, IL-3, IL-5, and IL-12 (p40) levels were lower in the diabetic group than in the control group. The TNF-α plasma level was significantly elevated in patients with proliferative diabetic retinopathy (PDR) compared with the levels in patients with non-proliferative diabetic retinopathy (NPDR) and patients with no apparent diabetic retinopathy (NDR).
TNF-α might be involved in the progression of DR, especially in the pathogenesis of PDR. TNF-α is a potential cytokine for the prognosis of DR and might act as a therapeutic target in DR.
本研究旨在评估血浆细胞因子在糖尿病视网膜病变（DR）中的作用及其与DR严重程度的关系。
本研究纳入了59例糖尿病患者和19例非糖尿病对照者。采用Luminex多重微珠免疫测定法检测血浆中内皮生长因子（EGF）、嗜酸性粒细胞趋化因子、成纤维细胞生长因子2（FGF-2）、Flt-3配体（Flt-3L）、 fractalkine、粒细胞集落刺激因子（G-CSF）、粒细胞巨噬细胞集落刺激因子（GM-CSF）、生长相关癌基因（GRO）、干扰素（IFN）-α2、IFN-γ、白细胞介素（IL）-1α、IL-1β、IL-1受体拮抗剂（IL-1Ra）、IL-2、IL-3、IL-4、IL-5、IL-6、IL-7、IL-8、IL-9、IL-10、IL-12（p40）、IL-12（p70）、IL-13、IL-15、IL-17、IFN诱导蛋白10（IP-10）、单核细胞趋化蛋白（MCP）-1、MCP-3、巨噬细胞衍生细胞因子（MDC）、巨噬细胞炎性蛋白（MIP）-1α、MIP-1β、可溶性CD40配体（sCD40L）、可溶性白细胞介素2受体α（sIL-2Rα）、转化生长因子（TGF）-α、肿瘤坏死因子（TNF）-α、TNF-β和血管内皮生长因子（VEGF）的浓度。根据DR分期研究这些细胞因子的水平。
与对照组相比，糖尿病组血浆中10种细胞因子——MCP-1、IL-6、IL-7,、IL-9、IL-13、IL-15、IL-17、sCD40L、sIL-2Rα和TNF-β水平显著升高。糖尿病组Flt-3L、IL-1Ra、IL-3、IL-5和IL-12（p40）水平低于对照组。与非增殖性糖尿病视网膜病变（NPDR）患者和无明显糖尿病视网膜病变（NDR）患者相比，增殖性糖尿病视网膜病变（PDR）患者血浆TNF-α水平显著升高。
TNF-α可能参与DR的进展，尤其是PDR的发病机制。TNF-α是DR预后的潜在细胞因子，可能成为DR的治疗靶点。
