Environmental Endocrinology and Biomedical Research Unit, Department of Zoology, Meerut College, Meerut 250003, India.
Scientific and Applied Research Center, Meerut 250001, India.
Evid Based Complement Alternat Med. 2014;2014:787153. doi: 10.1155/2014/787153. Epub 2014 Aug 31.
The goal of this study was to investigate the hepatoprotective effects of aqueous extract of Camellia sinensis or green tea extract (AQGTE) in chronic ethanol-induced albino rats. All animals were divided into 4 groups in the study for a 5-week duration. 50% ethanol was given orally to the rats with two doses (5 mg/kg bw and 10 mg/kg bw) of AQGTE. Ethanol administration caused a significant increase in the levels of plasma and serum enzymatic markers, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP), and nonenzymatic markers (cholesterol and triglycerides), lipid peroxidation contents, malondialdehyde (MDA), and glutathione-S-transferase (GST), and decreased the activities of total proteins, albumin, and cellular antioxidant defense enzymes such as superoxide dismutase (SOD). The elevation and reduction in these biochemical enzymes caused the damage in hepatocytes histologically due to the high production of ROS, which retards the antioxidant defense capacity of cell. AQGTE was capable of recovering the level of these markers and the damaged hepatocytes to their normal structures. These results support the suggestion that AQGTE was able to enhance hepatoprotective and antioxidant effects in vivo against ethanol-induced toxicity.
本研究旨在探讨茶绿茶水提物（AQGTE）对慢性乙醇诱导白化大鼠的保肝作用。在为期 5 周的研究中，所有动物分为 4 组。用两种剂量（5mg/kg bw 和 10mg/kg bw）的 AQGTE 给大鼠口服 50%乙醇。乙醇给药导致血浆和血清酶标记物丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和碱性磷酸酶（ALP）以及非酶标记物（胆固醇和甘油三酯）、脂质过氧化产物丙二醛（MDA）和谷胱甘肽-S-转移酶（GST）水平显著升高，总蛋白、白蛋白和细胞抗氧化防御酶如超氧化物歧化酶（SOD）的活性降低。这些生化酶的升高和降低导致肝细胞组织学损伤，因为 ROS 的产生增加，从而削弱了细胞的抗氧化防御能力。AQGTE 能够恢复这些标志物的水平，并使受损的肝细胞恢复正常结构。这些结果支持了 AQGTE 能够增强体内对抗乙醇诱导毒性的保肝和抗氧化作用的观点。
