Yang Jing, Zhen Le, Ren Fanghui, Campbell Jeremy, Rorrer Gregory L, Wang Alan X
School of Electrical Engineering and Computer Science, Oregon State University, Corvallis, 97331, USA.
School of Chemical, Biological & Environmental Engineering, Oregon State University, Corvallis, 97331, USA.
J Biophotonics. 2015 Aug;8(8):659-67. doi: 10.1002/jbio.201400070. Epub 2014 Sep 25.
We experimentally demonstrate an ultra-sensitive immunoassay biosensor using diatom biosilica with self-assembled plasmonic nanoparticles. As the nature-created photonic crystal structures, diatoms have been adopted to enhance surface plasmon resonances of metal nanoparticles on the surfaces of diatom frustules and to increase the sensitivity of surface-enhanced Raman scattering (SERS). In this study, a sandwich SERS immunoassay is developed based on the hybrid plasmonic-biosilica nanostructured materials that are functionalized with goat anti-mouse IgG. Our experimental results show that diatom frustules improve the detection limit of mouse IgG to 10 pg/mL, which is ˜100× better than conventional colloidal SERS sensors on flat glass. Ultra-sensitive immunoassay biosensor using diatom biosilica with self-assembled plasmonic nanoparticles.
我们通过实验展示了一种使用带有自组装等离子体纳米颗粒的硅藻生物硅的超灵敏免疫分析生物传感器。作为天然形成的光子晶体结构，硅藻已被用于增强硅藻壳表面金属纳米颗粒的表面等离子体共振，并提高表面增强拉曼散射（SERS）的灵敏度。在本研究中，基于用山羊抗小鼠IgG功能化的等离子体-生物硅杂化纳米结构材料开发了一种夹心SERS免疫分析方法。我们的实验结果表明，硅藻壳将小鼠IgG的检测限提高到了10 pg/mL，这比平板玻璃上的传统胶体SERS传感器要好约100倍。使用带有自组装等离子体纳米颗粒的硅藻生物硅的超灵敏免疫分析生物传感器。
