Kong Xianming, Li Erwen, Squire Kenny, Liu Ye, Wu Bo, Cheng Li-Jing, Wang Alan X
School of Electrical Engineering and Computer Science, Oregon State University, Corvallis, OR, 97331, USA.
J Biophotonics. 2017 Nov;10(11):1473-1484. doi: 10.1002/jbio.201700045. Epub 2017 May 9.
Diatomite consists of fossilized remains of ancient diatoms and is a type of naturally abundant photonic crystal biosilica with multiple unique physical and chemical functionalities. In this paper, we explored the fluidic properties of diatomite as the matrix for on-chip chromatography and, simultaneously, the photonic crystal effects to enhance the plasmonic resonances of metallic nanoparticles for surface-enhanced Raman scattering (SERS) biosensing. The plasmonic nanoparticle-decorated diatomite biosilica provides a lab-on-a-chip capability to separate and detect small molecules from mixture samples with ultra-high detection sensitivity down to 1 ppm. We demonstrate the significant potential for biomedical applications by screening toxins in real biofluid, achieving simultaneous label-free biosensing of phenethylamine and miR21cDNA in human plasma with unprecedented sensitivity and specificity. To the best of our knowledge, this is the first time demonstration to detect target molecules from real biofluids by on-chip chromatography-SERS techniques.
硅藻土由古代硅藻的化石遗骸组成，是一种天然丰富的光子晶体生物二氧化硅，具有多种独特的物理和化学功能。在本文中，我们探索了硅藻土作为芯片上色谱基质的流体特性，同时研究了光子晶体效应，以增强金属纳米颗粒的等离子体共振，用于表面增强拉曼散射（SERS）生物传感。装饰有等离子体纳米颗粒的硅藻土生物二氧化硅提供了一种芯片实验室功能，能够从混合样品中分离和检测小分子，检测灵敏度高达百万分之一。我们通过在实际生物流体中筛选毒素，以前所未有的灵敏度和特异性实现了人血浆中苯乙胺和miR21 cDNA的无标记生物传感，证明了其在生物医学应用中的巨大潜力。据我们所知，这是首次通过芯片上色谱-SERS技术从实际生物流体中检测目标分子的演示。
