Bak Jin Seop
Department of Chemical and Biomolecular Engineering, Advanced Biomass R&D Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701, Republic of Korea,
Biotechnol Lett. 2015 Feb;37(2):349-58. doi: 10.1007/s10529-014-1676-1. Epub 2014 Sep 26.
Dichomitus squalens, a wood-rotting fungus, can utilize recalcitrant biomass for growth; however, the extracellular metabolic processes involved are not well understood. A systematic target analysis of D. squalens has been carried out using optimized conditions for lignocellulolysis. Seven encoded proteins and 58 metabolites that consistently exhibited altered regulation patterns were identified. The predicted regulators were then vertically combined into a comprehensive network that included entire signaling pathways involved in D. squalens. Despite the diversity of these pathways, they showed complementary cooperation among themselves, ensuring the efficiency of active biodegradation and thereby yielding energy saving for the cells. Although several hydrolytic enzymes (e.g., polysaccharide-degrading enzymes, α/β-hydrolase, and peroxidases) were systematically utilized, peroxidative controllers (e.g., glyoxal oxidase and P450-oxidoreductase) were rarely generated.
密粘褶菌是一种木材腐朽真菌，能够利用难降解生物质进行生长；然而，其涉及的细胞外代谢过程尚未得到充分了解。利用优化的木质纤维素分解条件对密粘褶菌进行了系统的靶点分析。鉴定出了7种编码蛋白和58种代谢物，它们始终呈现出改变的调控模式。然后将预测的调控因子纵向整合到一个综合网络中，该网络包括密粘褶菌中涉及的整个信号通路。尽管这些通路具有多样性，但它们彼此之间表现出互补协作，确保了活性生物降解的效率，从而为细胞节省了能量。虽然系统地利用了几种水解酶（如多糖降解酶、α/β水解酶和过氧化物酶），但过氧化物调控因子（如乙二醛氧化酶和P450氧化还原酶）很少产生。
