Bak Jin Seop
Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701 Republic of Korea.
Springerplus. 2015 Feb 1;4:47. doi: 10.1186/s40064-015-0825-x. eCollection 2015.
To support the inefficient limitation of long-term biosystem by well-known simultaneous saccharification and fermentation (SSF), electron beam irradiated rice straw (at 80 kGy, 1 MeV, and 0.12 mA) was fermented using fungal-based simultaneous saccharification and fermentation (FBSSF) by saprophytic zygomycetes Mucor indicus. Based on the growth optimization (by response surface methodology), this eco-friendly bioprocess either without metabolic inhibitors (especially furfurals and acetic acids) or byproducts (especially glycerols) significantly increased the biodegradability and fermentability of lignocellulosic rice straw. Specifically, when irradiated straw was simultaneously bioconverted by M. indicus for 48 h, the ethanol yield was 57.2% of the theoretical maximum. This value was on the similar level as the 59.8% (for 144 h) measured from processed straw by well-known SSF. Furthermore, after FBSSF for 144 h based on large-scale mass balance, the ethanol concentration and production yield, and productivity were 34.6 g/L, 72.3% of the theoretical maximum, and 0.24 g/L/h, respectively.
为了通过著名的同步糖化发酵（SSF）来支持长期生物系统的低效限制，使用腐生接合菌印度毛霉通过基于真菌的同步糖化发酵（FBSSF）对电子束辐照的稻草（在80 kGy、1 MeV和0.12 mA条件下）进行发酵。基于生长优化（通过响应面法），这种无代谢抑制剂（尤其是糠醛和乙酸）或副产物（尤其是甘油）的环保生物过程显著提高了木质纤维素稻草的生物降解性和发酵性。具体而言，当辐照后的稻草被印度毛霉同步生物转化48小时时，乙醇产量为理论最大值的57.2%。该值与通过著名的SSF从处理后的稻草中测得的59.8%（144小时）处于相似水平。此外，基于大规模质量平衡进行144小时的FBSSF后，乙醇浓度、产率和生产率分别为34.6 g/L、理论最大值的72.3%和0.24 g/L/h。
