Kim Chung Sub, Kwon Oh Wook, Kim Sun Yeou, Choi Sang Un, Kim Ki Hyun, Lee Kang Ro
Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, 300 Chonchon-dong, Jangan-ku, Suwon, 440-746, Republic of Korea.
Lipids. 2014 Nov;49(11):1151-9. doi: 10.1007/s11745-014-3953-0. Epub 2014 Sep 26.
In the course of our continuing search for bioactive constituents of Korean medicinal plants, we isolated five new oxylipins, chaenomic acid A-E (1-5), and six known ones (6-11) from the twigs of Chaenomeles sinensis. The structures of the new compounds (1-5) were determined by spectroscopic methods, including 1D and 2D NMR ((1)H and (13)C NMR, (1)H-(1)H COSY, HMQC, HMBC, and NOESY), olefin cross-metathesis, and LC/MS analysis. The known compounds (6-11) were identified by comparison of their spectroscopic data and specific optical rotation with the reported data. The isolated compounds (1-11) were tested for their inhibitory effects on nitric oxide production in lipopolysaccharide-activated murine microglial cells and for their cytotoxic activities against four human cancer cell lines (A549, SK-OV-3, A498, and HCT-15).
在我们持续探索韩国药用植物生物活性成分的过程中，我们从中华木瓜的嫩枝中分离出了5种新的氧化脂质，即木瓜酸A - E（1 - 5），以及6种已知的氧化脂质（6 - 11）。通过光谱方法确定了新化合物（1 - 5）的结构，这些方法包括一维和二维核磁共振（¹H和¹³C NMR、¹H - ¹H COSY、HMQC、HMBC和NOESY）、烯烃交叉复分解反应以及液相色谱/质谱分析。通过将已知化合物（6 - 11）的光谱数据和比旋光度与已报道的数据进行比较来鉴定它们。测试了分离得到的化合物（1 - 11）对脂多糖激活的小鼠小胶质细胞中一氧化氮产生的抑制作用，以及它们对四种人类癌细胞系（A549、SK - OV - 3、A498和HCT - 15）的细胞毒性活性。
