Wei Yuxi, Liu Qi, Yu Jia, Feng Qiang, Zhao Ling, Song Huiping, Wang Wenxiu
a College of Life Sciences, Qingdao University , Qingdao 266071 , P.R. China.
Nat Prod Res. 2015;29(10):976-9. doi: 10.1080/14786419.2014.964705. Epub 2014 Sep 26.
As one kind of anthraquinone dihydroxy derivatives, 1,8-dihydroxy-anthraquinone (Dan) with strong antibacterial activity against Staphylococcus aureus was first isolated from Porphyra haitanensis. Here, we report on the investigation of the antibacterial mode of action of Dan on the Gram-positive bacterium S. aureus. The results show that Dan strongly inhibited cell growth at logarithmic phase. In this study, the antibacterial activity of Dan was analysed by using phosphorus standard solution, p-nitrophenyl phosphate, o-nitrophenyl-β-D-galactopyanoside, scanning electron microscopy and transmission electron microscopy. The results suggested that the antibacterial activity of Dan is due to its interaction with the cell wall and cell membrane, by which it increases the permeability of the cell envelope and leads to the leakage of cytoplasm and the deconstruction of cell. This study indicates that Dan as a natural product in seaweeds deserves further investigation for applications as an antibacterial bioactive substance in food safety control and drugs.
作为一种蒽醌二羟基衍生物，1,8 - 二羟基蒽醌（Dan）最初是从坛紫菜中分离出来的，它对金黄色葡萄球菌具有很强的抗菌活性。在此，我们报告了Dan对革兰氏阳性菌金黄色葡萄球菌的抗菌作用模式的研究。结果表明，Dan在对数生长期强烈抑制细胞生长。在本研究中，通过使用磷标准溶液、对硝基苯磷酸、邻硝基苯基 - β - D - 吡喃半乳糖苷、扫描电子显微镜和透射电子显微镜分析了Dan的抗菌活性。结果表明，Dan的抗菌活性归因于其与细胞壁和细胞膜的相互作用，通过这种作用增加了细胞包膜的通透性，导致细胞质泄漏和细胞解构。本研究表明，作为海藻中的一种天然产物，Dan作为一种抗菌生物活性物质在食品安全控制和药物方面的应用值得进一步研究。
