下一代测序技术获得了平头鲻（Mugil cephalus）隐秘物种NWP2（硬骨鱼纲：鲻科）的完整线粒体基因组。
Next generation sequencing yields the complete mitochondrial genome of the flathead mullet, Mugil cephalus cryptic species NWP2 (Teleostei: Mugilidae).
作者信息
Shen Kang-Ning, Yen Ta-Chi, Chen Ching-Hung, Li Huei-Ying, Chen Pei-Lung, Hsiao Chung-Der
机构信息
a Department of Environmental Biology and Fisheries Science , National Taiwan Ocean University , Keelung , Taiwan , ROC .
b Center of Excellence for the Oceans, National Taiwan Ocean University , Keelung , Taiwan , ROC .
出版信息
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1758-9. doi: 10.3109/19401736.2014.963799. Epub 2014 Sep 26.
In this study, the complete mitogenome sequence of Northwestern Pacific 2 (NWP2) cryptic species of flathead mullet, Mugil cephalus (Teleostei: Mugilidae) has been amplified by long-range PCR and sequenced by next-generation sequencing method. The assembled mitogenome, consisting of 16,686 bp, had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a non-coding control region of D-loop. D-loop was 909 bp length and was located between tRNA-Pro and tRNA-Phe. The overall base composition of NWP2 M. cephalus was 28.4% for A, 29.8% for C, 26.5% for T and 15.3% for G. The complete mitogenome may provide essential and important DNA molecular data for further phylogenetic and evolutionary analysis for flathead mullet species complex.
在本研究中，通过长距离PCR扩增了西北太平洋平头鲻（Mugil cephalus，硬骨鱼纲：鲻科）隐存种西北太平洋2号（NWP2）的完整线粒体基因组序列，并采用二代测序方法进行测序。组装后的线粒体基因组由16,686 bp组成，具有典型的脊椎动物线粒体基因排列，包括13个蛋白质编码基因、22个转运RNA、2个核糖体RNA基因和一个D环非编码控制区。D环长度为909 bp，位于tRNA-Pro和tRNA-Phe之间。NWP2平头鲻的总体碱基组成为：A占28.4%，C占29.8%，T占26.5%，G占15.3%。完整的线粒体基因组可为平头鲻物种复合体的进一步系统发育和进化分析提供重要的DNA分子数据。
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