下一代测序技术获得了大型珊瑚（刺胞动物门：鹿角珊瑚科）的完整线粒体基因组。
Next-generation sequencing yields the complete mitogenome of massive coral, (Cnidaria: Poritidae).
作者信息
Niu Wentao, Lin Rongcheng, Shi Xiaofeng, Chen Ching-Hung, Shen Kang-Ning, Hsiao Chung-Der
机构信息
Laboratory of Marine Biology and Ecology, Third Institute of Oceanography, State Oceanic Administration, Xiamen, China.
WeThink Biotech Inc., Taoyuan, Taiwan.
出版信息
Mitochondrial DNA B Resour. 2016 Feb 1;1(1):8-9. doi: 10.1080/23802359.2015.1137795.
In this study, the complete mitogenome sequence of massive coral, (Cnidaria: Poritidae), has been sequenced by next-generation sequencing method. The overall base composition of mitogenome is 26.0% for A, 13.3% for C, 23.0% for G and 37.8% for T and have high AT content of 63.7%. The assembled mitogenome, consisting of 18 646 bp, has unique 13 protein-coding genes (PCGs), seven transfer RNAs and two ribosomal RNAs genes. The mitogenome has the common mitogenome gene organization and feature of scleractinian coral. Among 13 PCGs, ND5 and COX1 genes are interrupted by group I intron (11 130 and 971 bp, respectively). There are 13 genes embedded in ND5 group I intron (tRNA-Glu, ND1, CYTB, tRNA-Met, ND2, ND6, ATP6, ND4, 12S rRNA, COX3, COX2, ND4L and ND3), and two genes embedded in COX1 group I intron (tRNA-Ile and tRNA-Pro). The complete mitogenome provides essential and important DNA molecular data for further phylogenetic and evolutionary analysis for stony coral.
在本研究中，通过下一代测序方法对块状珊瑚（刺胞动物门：孔珊瑚科）的完整线粒体基因组序列进行了测序。该线粒体基因组的总体碱基组成为：A占26.0%，C占13.3%，G占23.0%，T占37.8%，AT含量较高，为63.7%。组装后的线粒体基因组由18,646 bp组成，具有独特的13个蛋白质编码基因（PCGs）、7个转运RNA和2个核糖体RNA基因。该线粒体基因组具有石珊瑚常见的线粒体基因组基因组织和特征。在13个PCGs中，ND5和COX1基因被I组内含子打断（分别为11,130和971 bp）。ND5 I组内含子中嵌入了13个基因（tRNA-Glu、ND1、CYTB、tRNA-Met、ND2、ND6、ATP6、ND4、12S rRNA、COX3、COX2、ND4L和ND3），COX1 I组内含子中嵌入了2个基因（tRNA-Ile和tRNA-Pro）。完整的线粒体基因组为石珊瑚的进一步系统发育和进化分析提供了重要的DNA分子数据。
Zotero 插件