Chen Xiaowan, Sun Jingxu, Song Yongxi, Gao Peng, Zhao Junhua, Huang Xuanzhang, Liu Bo, Xu Huimian, Wang Zhenning
Department of Surgical Oncology and General Surgery, First Hospital of China Medical University, Shenyang, 110001, China.
Med Oncol. 2014 Nov;31(11):262. doi: 10.1007/s12032-014-0262-7. Epub 2014 Sep 27.
It is well known that the differential expression of long noncoding RNA (lncRNA) plays critical roles in carcinogenesis. However, the significance of lncRNA in the diagnosis of gastric cancer (GC) remains largely unknown. The goal of this study was to determine the expression level of AC138128.1, a novel lncRNA, in GC and its clinical association. The expression of AC138128.1 in 94 pairs of tumors and normal adjacent tissues (NAT) from patients with GC was detected by quantitative real-time reverse transcription polymerase chain reaction. The potential association between AC138128.1 expression level in GC tissue and clinicopathological features was analyzed. Finally, a receiver operating characteristic (ROC) curve was constructed. The results showed that the expression level of AC138128.1 in GC was significantly decreased compared to that in NAT (p < 0.001). Levels of AC138128.1 in the GC cell lines, AGS BGC-823, HG27, and SGC-7901 were lower than those in the human normal gastric epithelial cell line GES-1. Moreover, the area under the ROC curve was 0.688. This study demonstrates that lncRNA AC138128.1 might be a novel biomarker for predicting GC.
众所周知，长链非编码RNA（lncRNA）的差异表达在肿瘤发生过程中起着关键作用。然而，lncRNA在胃癌（GC）诊断中的意义仍 largely未知。本研究的目的是确定一种新型lncRNA即AC138128.1在GC中的表达水平及其临床相关性。通过定量实时逆转录聚合酶链反应检测了94对GC患者的肿瘤组织和癌旁正常组织（NAT）中AC138128.1的表达。分析了GC组织中AC138128.1表达水平与临床病理特征之间的潜在关联。最后，构建了受试者工作特征（ROC）曲线。结果显示，与NAT相比，GC中AC138128.1的表达水平显著降低（p < 0.001）。GC细胞系AGS、BGC - 823、HG27和SGC - 7901中AC138128.1的水平低于人正常胃上皮细胞系GES - 1。此外，ROC曲线下面积为0.688。本研究表明，lncRNA AC138128.1可能是预测GC的一种新型生物标志物。
