School of Life Sciences, NanoSafety Research Group, Heriot-Watt University, Edinburgh EH14 4AS, Scotland, UK.
School of Life Sciences, NanoSafety Research Group, Heriot-Watt University, Edinburgh EH14 4AS, Scotland, UK.
Environ Pollut. 2014 Dec;195:218-25. doi: 10.1016/j.envpol.2014.09.002. Epub 2014 Sep 27.
Bacteria based ecotoxicology assessment of manufactured nanoparticles is largely restricted to Escherichia coli bioreporters in laboratory media. Here, toxicity effects of model OECD nanoparticles (Ag NM-300K, ZnO NM-110 and TiO2 NM-104) were assessed using the switch-off luminescent Pseudomonas putida BS566::luxCDABE bioreporter in Luria Bertani (LB) medium and artificial wastewater (AW). IC50 values ∼4 mg L(-1), 100 mg L(-1) and >200 mg L(-1) at 1 h were observed in LB for Ag NM-300K, ZnO NM-110 and TiO2 NM-104, respectively. Similar results were obtained in AW for Ag NM-300K (IC50∼5 mg L(-1)) and TiO2 NM-104 (IC50>200 mg L(-1)) whereas ZnO NM-110 was significantly higher (IC50>200 mg L(-1)). Lower ZnO NM-110 toxicity in AW compared to LB was associated with differences in agglomeration status and dissolution rate. This work demonstrates the importance of nanoecotoxicological studies in environmentally relevant matrices.
基于细菌的纳米颗粒生态毒理学评估在很大程度上仅限于实验室培养基中的大肠杆菌生物报告器。在这里，使用发光假单胞菌 BS566::luxCDABE 生物报告器在 LB 培养基和人工废水 (AW) 中评估了模型 OECD 纳米颗粒 (Ag NM-300K、ZnO NM-110 和 TiO2 NM-104) 的毒性效应。在 LB 中，Ag NM-300K、ZnO NM-110 和 TiO2 NM-104 的 IC50 值分别为 ∼4 mg L(-1)、100 mg L(-1) 和 >200 mg L(-1)。在 AW 中也得到了类似的结果，Ag NM-300K (IC50∼5 mg L(-1)) 和 TiO2 NM-104 (IC50>200 mg L(-1)) 的 IC50 值也较高，而 ZnO NM-110 的 IC50 值则更高 (>200 mg L(-1))。与 LB 相比，AW 中的 ZnO NM-110 毒性较低，这与团聚状态和溶解速率的差异有关。这项工作证明了在环境相关基质中进行纳米生态毒理学研究的重要性。
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