Tannock G W, Dashkevicz M P, Feighner S D
Department of Microbiology, University of Otago, Dunedin, New Zealand.
Appl Environ Microbiol. 1989 Jul;55(7):1848-51. doi: 10.1128/aem.55.7.1848-1851.1989.
Mice that have a complex intestinal microflora but that do not harbor lactobacilli were used to determine the contribution of lactobacilli to the total bile salt hydrolase activity in the murine intestinal tract. Bile salt hydrolase activity in the ileal contents of these mice was reduced 86% in the absence of lactobacilli and by greater than 98% in the absence of lactobacilli and enterococci compared with samples from conventional mice. Bile salt hydrolase activities were lower in ileal and cecal contents from lactobacillus-free mice colonized with enterococci than in samples from lactobacillus-free mice colonized with lactobacilli. Bile salt hydrolase activity in the duodena, jejuna, ilea, and ceca of reconstituted lactobacillus-free mice colonized by lactobacilli was similar to that in samples from the intestinal tracts of conventional mice. We conclude from these studies that lactobacilli are the main contributors to total bile salt hydrolase activity in the murine intestinal tract.
具有复杂肠道微生物群落但不含乳酸杆菌的小鼠被用于确定乳酸杆菌对小鼠肠道中总胆汁盐水解酶活性的贡献。与常规小鼠的样本相比，这些小鼠回肠内容物中的胆汁盐水解酶活性在无乳酸杆菌时降低了86%，在无乳酸杆菌和肠球菌时降低了98%以上。用肠球菌定殖的无乳酸杆菌小鼠的回肠和盲肠内容物中的胆汁盐水解酶活性低于用乳酸杆菌定殖的无乳酸杆菌小鼠的样本。用乳酸杆菌定殖的重构无乳酸杆菌小鼠的十二指肠、空肠、回肠和盲肠中的胆汁盐水解酶活性与常规小鼠肠道样本中的相似。我们从这些研究中得出结论，乳酸杆菌是小鼠肠道中总胆汁盐水解酶活性的主要贡献者。
