Niyyati Maryam, Sasani Roghayeh, Mohebali Mehdi, Ghazikhansari Mahmoud, Kargar Faranak, Hajialilo Elham, Rezaeian Mostafa
Dept. of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Parasitol. 2018 Apr-Jun;13(2):180-185.
This study aimed to investigate the anti- effects of the most used marketed disinfecting solutions in Iran. Moreover, the efficacy of some nano-compounds was tested against pathogenic .
The present study was conducted in the School of Public Health, Tehran University of Medical sciences, Tehran, Iran during 2015-2016. Cysts of T4 genotype (7 × 10 /ml) mixed at the equal volume with contact lens solutions including Opti-free, Ginza, ReNu, Maxima, Light, and Cyclean for the recommended time by the manufacturers. Nano-silver and nano-gold compounds were also treated with the amoebae. Chlorhexidine 0.02% and normal saline were used as positive and negative controls, respectively. Dead and alive amoebae were determined using vital stain and suspension was cultured in non-nutrient agar. The entire process was repeated at least three times.
In none of the solutions in the manufacturer's brochure recommended time, full cytotoxic effect was observed on the cysts of . Opti free express solution destroyed the cysts after 6 days. Nanosilver and nano-gold compounds showed no cytotoxic effect on the cysts of .
None of the Nanoparticles compounds as well as contact lenses disinfecting solutions which studied was effective on cysts in the manufacturer's brochure recommended time. However, continuing study on Nano-silver and Nano-gold compounds to find effective ingredients against are highly recommended.
本研究旨在调查伊朗市场上最常用的消毒溶液的抗菌效果。此外，还测试了一些纳米化合物对病原体的功效。
本研究于2015 - 2016年在伊朗德黑兰医科大学公共卫生学院进行。将T4基因型的包囊（7×10/ml）与隐形眼镜护理液（包括Opti-free、Ginza、ReNu、Maxima、Light和Cyclean）按等体积混合，作用时间为制造商推荐的时间。纳米银和纳米金化合物也用于处理变形虫。0.02%的洗必泰和生理盐水分别用作阳性和阴性对照。使用活体染色法确定死的和活的变形虫，并将悬浮液接种于非营养琼脂中培养。整个过程至少重复三次。
在制造商手册推荐的时间内，没有一种溶液对包囊产生完全的细胞毒性作用。Opti free express护理液在6天后破坏了包囊。纳米银和纳米金化合物对包囊没有细胞毒性作用。
在制造商手册推荐的时间内，所研究的纳米颗粒化合物以及隐形眼镜消毒溶液对包囊均无效。然而，强烈建议继续研究纳米银和纳米金化合物，以找到对抗包囊的有效成分。
