Institut de Chimie pour les Procédés, l'Energie, l'Environnement et la Santé (ICPEES, UMR 7515 CNRS/Université de Strasbourg), 1 rue Blessig, 67084 Strasbourg Cedex, France.
Institut de Chimie pour les Procédés, l'Energie, l'Environnement et la Santé (ICPEES, UMR 7515 CNRS/Université de Strasbourg), 1 rue Blessig, 67084 Strasbourg Cedex, France; In'Air Solutions, 1 rue Blessig, 67000 Strasbourg, France.
Talanta. 2015 Jan;131:386-94. doi: 10.1016/j.talanta.2014.05.027. Epub 2014 Jun 4.
Most of Polycyclic Aromatic Hydrocarbons (PAHs) are associated to airborne particles and their health impact depends on the particle size where they are bound. This work aims to develop a high sensitive analytical technique to quantify particulate PAHs sampled with a 3-stages cascade impactor in order to derive simultaneously their individual concentration in PM1, PM2.5 and PM10. Three key steps of the method were evaluated separately in order to avoid any PAHs loss during the global sample preparation procedure: (1) the accelerated solvent extraction of PAHs from the filter; (2) the primary concentration of the extract until 1 mL by means of a rotary evaporator at 45°C and 220 mbar and (3) the final concentration of the pre-concentrated extract to about 100-150 µL under a gentle nitrogen stream. Each recovery experiment was realized in triplicates. All these steps evaluated independently show that the overall PAHs loss, even for those with a low molecular weight, should not exceed more than a few percent. Extracts were then analyzed by using a HPLC coupled to fluorescence and Diode Array Detectors with the external standard method. The resulting calibration curves containing between 9 and 12 points were plotted in the concentration range of 0.05-45 µg L(-1) for most of the 16 US-EPA priority PAHs and were fully linear (R(2)>0.999). Limits Of Quantification were in the range 0.05-0.47 µg L(-1) corresponding to 0.75-7.05 pg m(-3) for 20 m(3) of pumped air. Finally, taking into account the average PAHs concentrations previously reported in typical European indoor environments, and considering the use of a 3-stages cascade impactor to collect simultaneously PM>10 µm, 2.5 µm<PM<10 µm, 1 µm<PM<2.5 µm and PM<1 (and then to derive PM1, PM2.5 and PM10) for particle-bound PAHs quantification, the sampling duration was estimated to 20-40 h for a sampling flow fixed to 0.5m(3)h(-1).
大多数多环芳烃（PAHs）与空气颗粒物有关，其健康影响取决于它们结合的颗粒物粒径。本工作旨在开发一种高灵敏度的分析技术，以定量采集 3 级冲击式空气采样器采集的颗粒物中的 PAHs，以便同时得出它们在 PM1、PM2.5 和 PM10 中的各自浓度。为避免在整个样品制备过程中损失任何 PAHs，分别对方法的三个关键步骤进行了评估：（1）从滤纸上用加速溶剂萃取 PAHs；（2）在 45°C 和 220 mbar 的条件下，用旋转蒸发器将提取物浓缩至 1 mL；（3）在温和的氮气流下，将预浓缩提取物最终浓缩至约 100-150 µL。每个回收实验均重复三次。所有这些步骤的独立评估表明，即使对于那些低分子量的 PAHs，总损失也不应超过几个百分点。然后通过使用高效液相色谱法（HPLC）结合荧光和二极管阵列检测器，用外标法对提取物进行分析。在大多数情况下，16 种美国环保署优先 PAHs 的浓度范围为 0.05-45 µg L(-1)，包含 9-12 个点的校准曲线的线性度完全良好（R(2)>0.999）。定量下限（LOQ）在 0.05-0.47 µg L(-1)范围内，对应于 20 m(3)抽吸空气中的 0.75-7.05 pg m(-3)。最后，考虑到典型欧洲室内环境中先前报道的平均 PAHs 浓度，并考虑到使用 3 级冲击式空气采样器同时采集>10 µm、2.5 µm<PM<10 µm、1 µm<PM<2.5 µm 和 PM<1（然后推导 PM1、PM2.5 和 PM10）中的颗粒物结合 PAHs，采样时间估计为 20-40 小时，采样流量固定为 0.5 m(3)h(-1)。
