Department of Chemistry and Environmental Res Center, Missouri University of Science and Technology, Rolla MO, USA.
Department of Chemistry and Environmental Res Center, Missouri University of Science and Technology, Rolla MO, USA.
Talanta. 2015 Jan;131:736-41. doi: 10.1016/j.talanta.2014.08.003. Epub 2014 Aug 11.
N-Nitrosamines are potent mutagenic and carcinogenic emerging water disinfection by-products (DBPs). The most effective strategy to control the formation of these DBPs is minimizing their precursors from source water. Secondary and tertiary amines are dominating precursors of N-nitrosamines formation during drinking water disinfection process. Therefore, the screening and removal of these amines in source water are very essential for preventing the formation of N-nitrosamines. A rapid, simple, and sensitive ultrafast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) method has been developed in this study to determine seven amines, including dimethylamine, ethylmethylamine, diethylamine, dipropylamine, trimethylamine, 3-(dimethylaminomethyl)indole, and 4-dimethylaminoantipyrine, as major precursors of N-nitrosamines in drinking water system. No sample preparation process is needed except a simple filtration. Separation and detection can be achieved in 11 min per sample. The method detection limits of selected amines are ranging from 0.02 μg/L to 1 μg/L except EMA (5 μg/L), and good calibration linearity was achieved. The developed method was applied to determine the selected precursors in source water and drinking water samples collected from Midwest area of the United States. In most of water samples, the concentrations of selected precursors of N-nitrosamines were below their method detection limits. Dimethylamine was detected in some of water samples at the concentration up to 25.4 μg/L.
亚硝胺是一种强有力的诱变剂和致癌物质，是新兴的饮用水消毒副产物（DBP）。控制这些 DBP 形成的最有效策略是从源水中最大限度地减少其前体。仲胺和叔胺是饮用水消毒过程中亚硝胺形成的主要前体。因此，在源水中筛选和去除这些胺类物质对于防止亚硝胺的形成非常重要。本研究建立了一种快速、简单、灵敏的超快速液相色谱-串联质谱（UFLC-MS/MS）方法，用于测定饮用水系统中七种胺类物质，包括二甲胺、乙基亚胺、二乙胺、二丙胺、三甲胺、3-（二甲氨基甲基）吲哚和 4-二甲氨基安替比林，它们是亚硝胺的主要前体。除了需要进行简单的过滤外，不需要进行样品制备。每个样品的分离和检测可在 11 分钟内完成。除了 EMA（5μg/L）外，所选胺类的方法检测限在 0.02μg/L 至 1μg/L 之间，并且具有良好的校准线性度。该方法用于测定美国中西部地区水源水和饮用水样品中选定的前体。在大多数水样中，亚硝胺的选定前体浓度低于方法检测限。在一些水样中检测到二甲胺，浓度高达 25.4μg/L。
