Department of Biomedical Sciences, Sun Moon University, Asan 330-744, Korea.
Plant Pathol J. 2014 Sep;30(3):279-87. doi: 10.5423/PPJ.OA.01.2014.0002.
KYC 3262 was selected as a biocontrol agent against anthracnose on hot pepper from 813 extracts of myxobacterial isolates. Dual culture with Colletotrichum acutatum and 813 myxobacterial extracts was conducted, and 19 extracts were selected that inhibited germination and mycelial growth of C. acutatum. All selections were Sorangium cellulosum, which are cellulolytic myxobacteria from soil. With the infection bioassay on detached fruits in airtight containers, KYC 3262, KYC 3512, KYC 3279, and KYC 3584 were selected. The listed four myxobacteria were cultured in CSG/1 liquid media, and harvested filtrates were sprayed on the infected fruits. KYC 3262 was selected from the studies of attached fruit in a greenhouse study. KYC 3262 filtrate was applied for 3 years (from 2011 to 2013) in a field study in Asan, Republic of Korea. Control values of the KYC 3262 in the field were 31%, 89%, and 82% in 2011, 2012, and 2013, whereas values of the fungicide spray treatment were 19%, 97%, and 91%, respectively. Yields (kg/20 plants) of the KYC 3262 were 2.66 kg and 18.6 kg in 2011 and 2013, respectively, and those of the fungicide treatment were 2.0 kg and 20.2 kg, in 2011 and 2013, respectively.
从 813 个粘细菌分离物的提取物中，KYC3262 被选为防治辣椒炭疽病的生防菌。进行了炭疽病菌与 813 个粘细菌提取物的双层培养，选择了 19 个抑制炭疽病菌孢子萌发和菌丝生长的提取物。所有的选择都是来自土壤的纤维素分解粘细菌 Sorangium cellulosum。通过在密封容器中的离体果实上进行感染生物测定，选择了 KYC3262、KYC3512、KYC3279 和 KYC3584。列出的四种粘细菌在 CSG/1 液体培养基中培养，收获的滤液喷洒在感染的果实上。KYC3262 是从温室研究中的附着果实研究中选择的。KYC3262 滤液在韩国牙山市的田间研究中连续 3 年（2011 年至 2013 年）应用。KYC3262 在田间的控制值分别为 2011 年、2012 年和 2013 年的 31%、89%和 82%，而杀菌剂喷雾处理的数值分别为 19%、97%和 91%。KYC3262 的产量（每 20 株公斤）分别为 2011 年和 2013 年的 2.66 公斤和 18.6 公斤，而杀菌剂处理的产量分别为 2011 年和 2013 年的 2.0 公斤和 20.2 公斤。
