Giri Sidhartha, Kindo Anupma Jyoti
Wellcome Trust Research Laboratory, Department of Gastrointestinal Sciences, Christian Medical College, Vellore, Tamil Nadu, India.
Indian J Pathol Microbiol. 2014 Oct-Dec;57(4):595-7. doi: 10.4103/0377-4929.142680.
With the increase in invasive fungal infections due to Candida species and resistance to antifungal therapy, in vitro antifungal susceptibility testing is becoming an important part of clinical microbiology laboratories. Along with broth microdilution and disk diffusion method, various commercial methods are being increasingly used for antifungal susceptibility testing, especially in the developed world. In our study, we compared the antifungal susceptibility patterns of 39 isolates of Candida to three antifungal drugs (fluconazole, amphotericin B, ketoconazole) by Candifast and disk diffusion method. The following resistance pattern was found by Candifast: Fluconazole (30.8%), ketoconazole (12.8%), amphotericin B (0%). The results obtained by disk diffusion method were in complete agreement with Candifast results.
随着念珠菌属引起的侵袭性真菌感染增加以及对抗真菌治疗产生耐药性，体外抗真菌药敏试验正成为临床微生物实验室的重要组成部分。除了肉汤微量稀释法和纸片扩散法外，各种商业方法越来越多地用于抗真菌药敏试验，尤其是在发达国家。在我们的研究中，我们通过Candifast和纸片扩散法比较了39株念珠菌对三种抗真菌药物（氟康唑、两性霉素B、酮康唑）的抗真菌药敏模式。通过Candifast发现以下耐药模式：氟康唑（30.8%）、酮康唑（12.8%）、两性霉素B（0%）。纸片扩散法获得的结果与Candifast结果完全一致。
