Hoh Jian Meng, Dhanashree B
Department of Microbiology, Kasturba Medical College, Manipal University, Mangalore, India.
Department of Microbiology, Kasturba Medical College, Manipal University, Mangalore, India.
J Ayurveda Integr Med. 2017 Oct-Dec;8(4):233-237. doi: 10.1016/j.jaim.2017.04.009. Epub 2017 Aug 30.
Increase in resistance of Candida species, to routinely used antifungal agents has necessitated the quest for new drugs. Few studies have revealed that cow's urine can suppress the growth of pathogenic fungi. However there is no published report on antifungal effects of cow's urine on clinical Candida isolates.
The present study aims at exploring the antifungal potential of cow's urine on clinical isolates of Candida species.
In this in-vitro experimental study four standard strains and 37 clinical isolates of Candida species were tested for their susceptibility to amphotericin B, fluconazole and voriconazole, by disk diffusion method. Detection of MIC of cow's urine for the Candida isolates was done by agar dilution method using 20-50% concentration of cow's urine.
Clinical isolates of Candida albicans n = 22 (59.5%) Candida glabrata n = 6 (16.2%), Candida tropicalis n = 3 (8.1%) and other Candida species n = 6 were tested for their antifungal susceptibility. Among them, 18.9% were resistant to voriconazole, 24.3% to amphotericin B and 35.1% to fluconazole. Statistically significant association was observed between susceptibility of voriconazole and that of cow's urine (p = 0.045). C. albicans ATCC14053, Candida parapsilosis ATCC22019 and 75.7% of clinical isolates of Candida were susceptible to cow's urine.
Cow's urine distillate has concentration dependent inhibitory effect on Candida species and is effective on the isolates that are either resistant or sensitive to the routinely used antifungal agents.
念珠菌属对常用抗真菌药物的耐药性增加，因此需要寻找新药。很少有研究表明牛尿可以抑制致病真菌的生长。然而，尚无关于牛尿对临床念珠菌分离株抗真菌作用的报道。
本研究旨在探索牛尿对念珠菌临床分离株的抗真菌潜力。
在这项体外实验研究中，通过纸片扩散法检测了4株念珠菌标准菌株和37株临床分离株对两性霉素B、氟康唑和伏立康唑的敏感性。采用琼脂稀释法，使用浓度为20%-50%的牛尿检测牛尿对念珠菌分离株的最低抑菌浓度（MIC）。
对白色念珠菌临床分离株n = 22（59.5%）、光滑念珠菌n = 6（16.2%）、热带念珠菌n = 3（8.1%）和其他念珠菌属n = 6进行了抗真菌药敏试验。其中，18.9%对伏立康唑耐药，24.3%对两性霉素B耐药，35.1%对氟康唑耐药。伏立康唑敏感性与牛尿敏感性之间存在统计学显著相关性（p = 0.045）。白色念珠菌ATCC14053、近平滑念珠菌ATCC22019以及75.7%的念珠菌临床分离株对牛尿敏感。
牛尿馏出物对念珠菌属具有浓度依赖性抑制作用，对常规使用的抗真菌药物耐药或敏感的分离株均有效。
