Tian Liran, Song Xiangwei, Liu Tianjiao, Li Anfeng, Ning Yang, Hua Xiuyi, Liang Dapeng
Key Laboratory of Groundwater Resources and Environment of Ministry of Education, College of New Energy and Environment, Jilin University, Changchun, 130012, People's Republic of China.
School of Life Sciences, Changchun Normal University, Changchun, 130031, People's Republic of China.
Anal Sci. 2022 Oct;38(10):1247-1259. doi: 10.1007/s44211-022-00168-6. Epub 2022 Aug 5.
The issue of environmental pollutant residues has gained wide public attention all along. Therefore, it is necessary to develop simple, rapid, economical, portable, and sensitive detection techniques, which have become the focus of research in the pollutants detection field. Spectroscopy is one of the most convenient, simple, rapid, and intuitive analytical tools that can provide accurate information, such as ultraviolet spectroscopy, fluorescence spectroscopy, Raman spectroscopy, plasmon resonance spectroscopy, etc. Gold nanoparticles, silver nanoparticles, and their dimers with unique optical properties are commonly used in the construction of spectroscopic probes. As a class of oligonucleotides that can recognize specific target molecules, aptamers also have a strong ability to recognize small-molecule pollutants. The application of aptamer-binding metal nanoparticles in biosensing detection presents significant advantages for instance high sensitivity, good selectivity, and rapid analysis. And many spectroscopic probes constructed by aptamer-binding gold nanoparticles, silver nanoparticles, or their dimers have been successfully demonstrated for detecting pollutants. This review summarizes the progress, advantages, and disadvantages of aptamer sensing techniques constructed by visual colorimetric, fluorescence, Raman, and plasmon resonance spectroscopic probes combining gold/silver nanoparticles or their dimers in the field of pollutants detection, and discusses the prospects and challenges for their future.
环境污染物残留问题一直备受公众广泛关注。因此，有必要开发简单、快速、经济、便携且灵敏的检测技术，这已成为污染物检测领域的研究热点。光谱学是最便捷、简单、快速且直观的分析工具之一，能提供准确信息，如紫外光谱、荧光光谱、拉曼光谱、等离子体共振光谱等。具有独特光学性质的金纳米粒子、银纳米粒子及其二聚体常用于构建光谱探针。适体作为一类能识别特定靶分子的寡核苷酸，对小分子污染物也有很强的识别能力。适体结合金属纳米粒子在生物传感检测中的应用具有显著优势，如高灵敏度、良好的选择性和快速分析等。许多由适体结合金纳米粒子、银纳米粒子或其二聚体构建的光谱探针已成功用于检测污染物。本文综述了在污染物检测领域，通过可视比色、荧光、拉曼和等离子体共振光谱探针结合金/银纳米粒子或其二聚体构建的适体传感技术的进展、优缺点，并探讨了其未来的前景与挑战。
