Shi Meisen, Liu Yaju, Bai Rufeng, Jiang Lizhe, Lv Xiaojiao, Ma Shuhua
Department of Radiology, First Affiliated Hospital, Medical College of Shantou University, Shantou, China.
Int J Legal Med. 2015 Jan;129(1):53-6. doi: 10.1007/s00414-014-1091-0. Epub 2014 Oct 14.
In this study, we assessed 30 insertion-deletion polymorphisms (Indels) (Investigator DIPplex® kit) in four Chinese populations (n = 952) and evaluated their usefulness in forensic genetic applications. After the Bonferroni correction at a 95 % significance level (p = 0.0017), there were no deviations from the Hardy-Weinberg equilibrium observed except for the HLD114 locus in the Tibetan ethnic group studied. A high level of discrimination power was observed for the DIPplex® kit in four sample populations (CDP > 0.9999) and the combined random match probabilities (CMP) were in the range of 1.4766 × 10(-11) to 5.2742 × 10(-13). Four Indels have been selected for further analyses as possible ancestry informative single nucleotide polymorphisms. The study support that the Investigator DIPplex® kit provides a powerful supplement to standard short tandem repeat-based kits for individual identification and kinship analysis in the Chinese population.
在本研究中，我们评估了四个中国人群（n = 952）中的30个插入缺失多态性（Indels）（Investigator DIPplex®试剂盒），并评估了它们在法医遗传学应用中的效用。在95%显著性水平（p = 0.0017）进行Bonferroni校正后，除了所研究的藏族人群中的HLD114位点外，未观察到偏离哈迪-温伯格平衡的情况。在四个样本群体中观察到DIPplex®试剂盒具有较高的鉴别力（CDP > 0.9999），并且联合随机匹配概率（CMP）在1.4766×10^(-11)至5.2742×10^(-13)范围内。已选择四个Indels作为可能的祖先信息单核苷酸多态性进行进一步分析。该研究支持Investigator DIPplex®试剂盒为中国人群个体识别和亲属关系分析的基于标准短串联重复序列的试剂盒提供了有力补充。
