Institute of Forensic Medicine, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China.
Institute of Forensic Medicine, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China.
Forensic Sci Int Genet. 2019 May;40:e219-e227. doi: 10.1016/j.fsigen.2019.02.002. Epub 2019 Feb 5.
Insertion/deletion analysis can serve as a promising and useful supporting tool in forensic research. The Qiagen Investigator® DIPplex Kit contained 30 well-chosen autosomal InDels was targeted to reveal the population genetic variation. In the present study, 10 Tibetan populations residing in different geographic areas of China were recruited and genotyped by Investigator® DIPplex Kit. Allele frequencies and forensic parameters were determined. No significant departures from Hard-Weinberg equilibrium (HWE) in all loci/populations after Bonferroni correction. The combined matching probability values range from 1.7148 × 10 to 5.3516 × 10° in 10 Tibetan populations. Our results revealed 10 Tibetan populations in China are genetically very similar. Intercontinental population differentiation analysis indicated Tibetan populations had a close genetic relationship with East Asian populations using Hierarchical clustering, multi-dimensional scaling (MDS), principal component analysis (PCA) and STRUCTURE. Comprehensive population genetic studies revealed that the 30-InDels assay was similarly efficient in forensic personal identification and could be regarded as an effective supplementary protocol for kinship testing.
插入/缺失分析可以作为法医学研究中一种有前途且有用的辅助工具。Qiagen Investigator® DIPplex 试剂盒包含 30 个经过精心挑选的常染色体 InDels，旨在揭示群体遗传变异。在本研究中，我们招募了居住在中国不同地理区域的 10 个藏族人群，并使用 Investigator® DIPplex 试剂盒对其进行基因分型。确定了等位基因频率和法医学参数。经 Bonferroni 校正后，所有位点/人群均未偏离 Hardy-Weinberg 平衡（HWE）。在中国的 10 个藏族人群中，联合匹配概率值范围从 1.7148×10-9 到 5.3516×10-9。我们的结果表明，中国的 10 个藏族人群在遗传上非常相似。洲际人群分化分析表明，使用层次聚类、多维尺度分析（MDS）、主成分分析（PCA）和 STRUCTURE，藏族人群与东亚人群具有密切的遗传关系。综合的群体遗传学研究表明，30 个 InDels 检测在法医学个体识别中同样有效，可以作为亲权鉴定的有效补充方案。
