Department of Bioactive Material Sciences and Research Center of Bioactive Materials, Chonbuk National University, Jeonju, 561-756, Korea.
J Plant Res. 2015 Jan;128(1):187-99. doi: 10.1007/s10265-014-0665-8. Epub 2014 Oct 15.
The circadian clock is a biological time keeper mechanism that regulates biological rhythms to a period of approximately 24 h. The circadian clock enables organisms to anticipate environmental cycles and coordinates internal cellular physiology with external environmental cues. In plants, correct matching of the clock with the environment confers fitness advantages to plant survival and reproduction. Therefore, circadian clock components are regulated at multiple layers to fine-tune the circadian oscillation. Epigenetic regulation provides an additional layer of circadian control. However, little is known about which chromatin remodeling factors are responsible for circadian control. In this work, we analyzed circadian expression of 109 chromatin remodeling factor genes and identified 17 genes that display circadian oscillation. In addition, we also found that a candidate interacts with a core clock component, supporting that clock activity is regulated in part by chromatin modification. As an initial attempt to elucidate the relationship between chromatin modification and circadian oscillation, we identified novel regulatory candidates that provide a platform for future investigations of chromatin regulation of the circadian clock.
生物钟是一种生物计时机制，它将生物节律调节到大约 24 小时的周期。生物钟使生物体能够预测环境周期，并将细胞内生理与外部环境线索协调起来。在植物中，时钟与环境的正确匹配赋予了植物生存和繁殖的优势。因此，生物钟组件在多个层面上受到调节，以微调生物钟振荡。表观遗传调控提供了生物钟控制的另一个层面。然而，对于哪些染色质重塑因子负责生物钟控制，我们知之甚少。在这项工作中，我们分析了 109 个染色质重塑因子基因的昼夜表达，鉴定出 17 个显示昼夜振荡的基因。此外，我们还发现一个候选基因与一个核心时钟组件相互作用，支持时钟活性部分受到染色质修饰的调节。作为阐明染色质修饰与昼夜振荡之间关系的初步尝试，我们确定了新的调控候选基因，为未来对生物钟的染色质调控的研究提供了一个平台。
