Department of Biological Sciences, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
Planta. 2018 Aug;248(2):513-518. doi: 10.1007/s00425-018-2921-y. Epub 2018 May 22.
The histone acetyltransferase HAF2 facilitates H3 acetylation deposition at the PRR5 and LUX promoters to contribute to robust circadian oscillation. The circadian clock ensures synchronization of endogenous rhythmic processes with environmental cycles. Multi-layered regulation underlies precise circadian oscillation, and epigenetic regulation is emerging as a crucial scheme for robust circadian maintenance. Here, we report that HISTONE ACETYLTRANSFERASE OF THE TAFII250 FAMILY 2 (HAF2) is involved in circadian homeostasis. The HAF2 gene is activated at midday, and its temporal expression is shaped by CIRCADIAN CLOCK-ASSOCIATED 1. The midday-activated HAF2 protein stimulates H3 acetylation (H3ac) deposition at the PRR5 and LUX loci, contributing to establishment of the raising phase. These results indicate that epigenetic waves in circadian networks underlie temporal compartmentalization of circadian components and stable maintenance of circadian oscillation.
组蛋白乙酰转移酶 HAF2 有助于 PRR5 和 LUX 启动子处 H3 乙酰化沉积，从而促进节律性振荡。生物钟确保内源性节律过程与环境周期同步。精确的昼夜节律振荡受到多层次的调节，表观遗传调节正在成为稳健的生物钟维持的关键方案。在这里，我们报告 HISTONE ACETYLTRANSFERASE OF THE TAFII250 FAMILY 2 (HAF2) 参与生物钟稳态。HAF2 基因在中午被激活，其时间表达受 CIRCADIAN CLOCK-ASSOCIATED 1 的影响。中午激活的 HAF2 蛋白刺激 PRR5 和 LUX 基因座处的 H3 乙酰化（H3ac）沉积，有助于建立上升阶段。这些结果表明，昼夜节律网络中的表观遗传波是昼夜节律成分的时间分隔和昼夜节律振荡的稳定维持的基础。
